波形蛋白在子痫前期蜕膜组织中的表达及其意义  

Expression of Vimentin in Decidua Tissues of Preeclampsia Patients and Its Significance

作　　者：冯亚玲[1] 周昌菊[1] 唐华贵 

机构地区：[1]中南大学湘雅医学院第三附属医院妇产科,长沙410013 [2]深圳市宝安区福永医院,深圳518103

出　　处：《生殖与避孕》2008年第8期477-481,490,共6页Reproduction and Contraception

摘　　要：目的:探讨波形蛋白在子痫前期蜕膜组织中的表达及意义。方法:采用荧光实时定量PCR(SYBR GREEN I assay)、Western blotting和免疫组织化学技术分别对晚发型重度子痫前期(A组)及早发型重度子痫前期(B组)和正常足月妊娠妇女(对照组,C组)各10例的波形蛋白mRNA及蛋白进行测定。结果:波形蛋白主要表达于蜕膜细胞的胞膜、胞浆及间质。荧光实时定量PCR,Western blotting和免疫组化表达等各方法验证结果显示:波形蛋白mRNA及蛋白在C组蜕膜组织中表达最高;B组中次之;A组中最低,各组间差异有统计学意义(P<0.05)。结论:波形蛋白表达下调可能参与子痫前期的病理过程,并且波形蛋白下调的程度同子痫前期发病的早晚有关。Objective： To investigate the expression of vimentin in the decidua tissues of preeclampsia. Methods： Using the technique of real time PCR, Western blotting and immunohistochemistry, the mRNA and protein expression of vimentin were measured in deciduas from 10 early onset severe preeclampsia patients （group A）, 10 late onset severe preeclampsia patients （group B） and 10 normal women of full-term pregnancy （group C）. Results： Vimentin was showed in cell membrane, endochylema ofdecidual cell and stroma. Both the mRNA and the protein of vimentin expressed in decidua tissues were significantly different among three groups, respectively （P〈0.05）. The expressional level of vimentin in group C was the highest, group A and group B. Conclusion： The lower expression of vimentin in the decidua tissues of preeclampsia might play a crucial role for the pathogenesis and the progress of preeclampsia.

关 键 词：子痫前期 波形蛋白 免疫组化 荧光实时定量PCR 免疫印迹 

分 类 号：R714.244[医药卫生—妇产科学]