丹参酮ⅡA对鼠脑缺血再灌注损伤Bcl-2和caspase-3表达的影响  

Effects of Tanshinone ⅡA on expressions of Bcl-2 and caspase-3 following cerebral ischemic reperfusion injury in rats

作　　者：李浩[1] 刘开祥[1] 俸军林[1] 唐永刚[1] 蒋静子[1] 林小慧[1] 

机构地区：[1]桂林医学院附属医院,广西桂林541002

出　　处：《中国康复医学杂志》2008年第8期691-693,I0002,共4页Chinese Journal of Rehabilitation Medicine

基　　金：广西自然科学基金资助(编号0542110)

摘　　要：目的:探讨丹参酮ⅡA(Tan ⅡA)对缺血再灌注损伤大鼠Bcl-2及caspase-3蛋白表达的影响。方法:将大鼠随机分为假手术组、缺血再灌注组、Tan ⅡA低剂量治疗组和Tan ⅡA高剂量治疗组,线栓法建立局灶性脑缺血再灌注模型。Tan ⅡA高、低剂量治疗组于术前连续灌胃给予高、低剂量Tan ⅡA3d,每天1次。用免疫组化法观察缺血90min再灌注24h大鼠额顶部皮质Bcl-2和caspase-3蛋白表达,并进行2,3,5-三苯基氯化四氮唑染色和HE染色观察脑梗死体积及病理形态学变化。结果:脑缺血再灌注24h,缺血再灌注组Bcl-2和caspase-3表达增加,与假手术组比较,差异具有显著性意义(P<0.05);与缺血再灌注组比较,Tan ⅡA高、低剂量治疗组均显著增加Bcl-2表达,减少caspase-3表达,高、低剂量组之间差异亦具有显著性意义(P<0.05)。Tan ⅡA高、低剂量治疗组脑梗死体积较缺血再灌注组减小,高、低剂量组之间差异具有显著性意义(P<0.05)。Tan ⅡA高、低剂量治疗组脑组织缺血损伤病理学改变明显轻于缺血再灌注组,TanⅡA高剂量治疗组缺血改变亦轻于低剂量治疗组。结论:Tan ⅡA对缺血再灌注脑损伤具有保护作用,其机制可能与增加Bcl-2蛋白表达同时降低caspase-3蛋白表达有关。Objective：To study the effects of Tanshinone Ⅱ A（Tan Ⅱ A） on expressions of Bcl-2 and caspase-3 of cerebral ischemia reperfusion（I/R） injury in rats.Method： Rats were randomly divided into four groups, which were sham operated group （SG）, I/R group, low dose Tan Ⅱ A treated group （LG） and high dose Tan Ⅱ A treated group （HG）. The focal middle cerebral artery occlusion （MCAO） model was made in rats with suture-occluded method except SG group. LG and HG groups were pretreated with low and high does Tan Ⅱ A, for 3d, before MCAO. After 90min MCAO following 24h reperfusion, expressions of Bc1-2 and easpase-3 in cerebral cortex were investigated with immunohistoehemistry technique. Result： Compared with SG group, in I/R group expressions of Bcl-2 and caspase-3 elevated at 24h reperfusion in ischemic region （P〈0.05）. Compared with I/R group, in LG and HG groups expression of Bcl-2 elevated dose-dependently and expressions of caspase-3 reduced dose-dependently （P〈0.05）. Compared with I/R group, in LG and HG groups cerebral infarction volumes decreased dose-dependently （P〈0.05）. In LG and HG groups changes of cerebral ischemic impairment were lighter than that in I/R group, and in HG group the changes were lighter than that in LG group. Conclusion：Tan Ⅱ A may reduced cerebral ischemia-reperfusion injury by elevating the expressions of Bcl-2 and reducing the expression of caspase-3. It plays protective effect on cerebral ischemia injury.

关 键 词：脑缺血再灌注 BCL-2 CASPASE-3 丹参酮 

分 类 号：R285.5[医药卫生—中药学]