大鼠海马神经干细胞体外培养分化过程中钾电流的变化  

THE CHANGE OF POTASSIUM CURRENT OF NEURAL STEM CELLS CULTRURED IN VITRO FROM NEWBORN RAT HIPPOCAMPUS

作　　者：邢莹[1] 张紫娟[1] 景莹[2] 韩雪飞[1] 许燕[1] 鄢文海[1] 

机构地区：[1]郑州大学医学院干细胞研究中心 [2]郑州大学医学院生理教研室,河南郑州450052

出　　处：《中国应用生理学杂志》2008年第3期306-309,共4页Chinese Journal of Applied Physiology

基　　金：河南省科技攻关基金资助项目(0424410085);河南省医学科技创新人才工程项目(2004013)

摘　　要：目的:探讨新生大鼠海马神经干细胞体外培养分化后的神经元样细胞钾电流的变化。方法:神经干细胞体外扩增培养并传代后,撤除有丝分裂原并加血清诱导分化,应用全细胞电压钳技术检测分化后培养1d、7d、14d、21d细胞的电压依赖性钾电流。结果:分化后培养1d的细胞,未检测出钾电流;分化后培养7d、14d、21d的细胞,在+50mV电压水平下的钾电流幅值分别为(18.077±2.789)pA/pF,(13.099±2.742)pA/pF,(34.045±8.067)pA/pF。该电流为两种电流的混合,分别能被TEA和4-AP所阻断,可能为缓慢失活的延迟整流钾电流(IK)和快速失活的瞬时外向钾电流(IA)。结论:新生大鼠海马神经干细胞诱导分化后,随着体外培养时间的延长,钾离子通道的功能逐渐成熟。Aim： To observe the change of potassium current on cultured neurons differentiated from hippocampus neural stem cells of the newborn rat. Methods： Neural stem cells from newborn rat hippocampus were cultured in vitro and passaged continuously. Differentiation of the cell was induced by serum and removing mitogens. After differentiation cells were plated on plastic dishes and cultured for 1 d, 7 d, 14 d and 21 d. Whole-cell voltage patch clamp recording was used respectively to detect voltage-dependent K^ ＋ current. Results： After 1 d culture, no current was detected, and on the 7^th d, 14^th d, 21^st d after differentiation, the amplitude of K^＋ currents was （18. 077 ± 2. 789）pA/pF, （13. 099 ± 2. 742）pA/pF, （34.045±8.067）pA/pFat ＋50 mV. The recorded K＋ current included two components that could be blocked by TEA and 4-AP separately, assumed the slowly inactivating delayed rectifier K^ ＋ current（ IK） and the fast inactivating transient outward K^＋ current（IA）. Conclusion： The function of potassium channels on the hippocampus neural stem cells of the newborn rat approaches mature gradually when the time of differentiation becomes longer in vitro.

关 键 词：神经干细胞 延迟整流钾电流(IK) 瞬时外向钾电流(IA) 

分 类 号：R331[医药卫生—人体生理学]