小脑间位核对淋巴细胞功能的调节作用  

EFFECT OF CEREBELLAR INTERPOSED NUCLEI ON LYMPHOCYTE FUNCTION

作　　者：邱健[1] 彭聿平[1] 邱一华[1] 

机构地区：[1]南通大学医学院生理学教研室

出　　处：《中国应用生理学杂志》2008年第3期310-314,共5页Chinese Journal of Applied Physiology

基　　金：江苏省“六大人才高峰”项目(06-B-040);江苏高等学校优秀科技创新团队项目(2007-5);江苏省卫生科研项目(H200761)

摘　　要：目的:研究小脑深部核团之一间位核对淋巴细胞功能的调节作用,以拓宽对小脑功能的认识进而增加神经免疫学的知识。方法:在大鼠双侧小脑间位核内注射海人酸(KA)以损毁间位核内神经元的胞体,并设对照组,于小脑间位核内注入等量生理盐水。在手术后的第8、16、32d分别用血细胞计数法检测动物外周血中淋巴细胞的数量;用四甲基偶氮唑(MTT)比色法检测动物肠系膜淋巴结细胞对刀豆蛋白A(ConA)刺激的增殖反应;用ELISA法检测动物血清中抗绵羊红细胞(SRBC)特异性IgM抗体的生成能力;用流式细胞术测定脾脏自然杀伤(NK)细胞的活性。结果:小脑间位核损毁后的第8、16、32d,动物外周血中淋巴细胞数都明显低于损毁手术前的淋巴细胞数,也显著低于生理盐水对照组相应时间段的淋巴细胞数。在小脑间位核注射KA后的第8、16、32d,动物的肠系膜淋巴结细胞由ConA诱导的增殖反应、血清中特异性抗SRBCIgM抗体的生成能力和脾脏NK细胞杀伤靶细胞YAC-1的活性均明显低于生理盐水对照组,但比较损毁后不同时间段的T、B和NK细胞功能的变化,没有发现显著的差异。结论:小脑双侧间位核损毁可导致总淋巴细胞数以及T、B和NK细胞功能均发生不可逆的降低,充分说明小脑间位核可调节淋巴细胞的功能,并提示在正常体内,小脑间位核对淋巴细胞功能具有增强效应。Aim： To increase the cognition of cerebellar functions and the knowledge of neuroimmunology, the effect of cerebellar interposed nuclei（IN）, one of three deep nuclei in cerebellum, on lymphocyte function was investigated. Methyls： Kainic acid（KA） was mi croinjeeted into bilateral IN for lesions of neuronal bodies in the IN. Control rats was mieroinjected with saline into their IN. On days 8, 16 and 32 following the IN lesions, the lymphocyte number in the peripheral blood was measured by blood corpuscle counter. Meanwhile, lymphocyte proliferation induced by concanavalin A （Con A）, cytotoxicity of natural killer （NK） cells against YAC- 1 cells, and anti-SRBC IgM antibody in the serum were examined respectively by methyl-thiazole-tetrazolium（MTT） assay, flow cytometry and ELISA assay. Results： The lymphocyte number in the peripheral blood was significantly reduced on days 8, 16 and 32 following the effective lesions of the bilateral IN in comparison with that of control. The COn A-induced lymphocyte proliferation, the NK cell cytotoxicity to YAC-I cells, and the titer of anti-SRBC lgM antibody in the serum, were all significantly attenuated on days 8, 16 and 32 following the effective lesions of the bilateral IN in comparison with those of control. There were not remarkable differences between the days 8, 16 and 32 in the decreased lymphocyte number and functions induced by the lesions of the bilateral IN. Conclusion： Effective lesions of the cerebellar bilaleral IN of rats cause an inhibition in lymphocyte number and functions of T, B and NK cells, strongly showing that the cerebellar IN ean modulate lymphocyte functions.

关 键 词：小脑 间位核 淋巴细胞 海人酸 神经免疫调节 

分 类 号：R338[医药卫生—人体生理学]