DIGFA与FQ-PCR法联合检测患儿肺炎支原体  

Detecting mycoplasma pneumoniae by fluorogenic quantitative polymerase chain reaction and dot immunogold filtration assay

作　　者：喻海忠[1] 袁建芬[1] 

机构地区：[1]江苏省南通市中医院,江苏南通226001

出　　处：《现代中西医结合杂志》2008年第27期4225-4226,共2页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的探索一种检测患儿肺炎支原体(MP)的测定方法。方法采用ELISA法、斑点金免疫渗滤法(DIGFA)和实时荧光定量PCR(FQ-PCR)技术分别测定患儿的MP。结果在71例培养或补体结合试验(CFT)阳性的标本中FQ-PCR阳性率为92%(65/71),DIGFA法MP-IgM阳性率为72%(51/71),ELISA法MP-IgM阳性率为70%(50/71)。FQ-PCR阳性率与ELISA和DIGFA二法比较有显著性差异(P均<0.01);DIGFA法阳性率和ELISA法比较无显著性差异(P>0.05)。培养或CFT阴性的43例咳嗽发热患者MP均为阴性。结论DIGFA联合FQ-PCR检测肺炎支原体具有快速、方便、准确的特点,可以满足临床的需要。Objective It is to approach a method for measuring mycoplasma pneumoniae （MP） from suffer children, Methods MP was detected respective with fluorogenic quantitative polyrnerase chain reaction （FQ- PCR）、 dot immunogold filtration assay （DIGFA） and enzyme linked immunosorbent assay （ELISA）, Results In 71 patients with MP infection diagnosed by sputum culture and/or complement fixation test, the positive rate of FQ - PCR, DIGFA and ELISA were 92 % （65/71）, 72% （51/71） and 70% （50/71） respective. There was significant difference between FQ- PCR and ELISA or DIGFA （P〈 0.01）. There was no significant difference between DIGFA and ELISA （P 〉0.05）, In 43 patients without MP infection was all negative. Conclusion FQ- PCR combining with DIGFA for detecting MP is a timesaving, sensitive and specific assay and can satisfy clinical require for diagnosing MP infection.

关 键 词：聚合酶链反应 肺炎支原体 细菌 斑点金免疫渗滤法 

分 类 号：R378.99[医药卫生—病原生物学] R725.631[医药卫生—基础医学]