同源四倍体多胚苗水稻双受精过程及胚胎早期发育的观察  

Observation on the Double Fertilization and Early Stage Development of Embryo in Autotetraploid Polyembryonic Rice

作　　者：代西梅[1] 黄群策[1] 梁秋霞[1] 秦广雍[1] 

机构地区：[1]郑州大学离子束生物工程河南省重点实验室,河南郑州450052

出　　处：《中国水稻科学》2008年第5期493-498,共6页Chinese Journal of Rice Science

基　　金：国家"十五"科技攻关资助项目(2001BA302B)

摘　　要：以同源四倍体多胚苗突变水稻IR36-双为材料,利用激光扫描共聚焦显微镜技术对其双受精过程及胚、胚乳的早期发育特征进行了观察。在"IR36-双"双受精及胚胎发育过程中,观察到一些异常受精现象和胚囊发生败育的异常类型。统计结果表明,异常受精作用或败育胚囊的发生频率高达46.67%,远高于其野生型品种IR36-4x(33.00%)。主要有子房退化、胚囊退化、单受精、双胚珠、双胚等几种异常类型。这些异常类型是导致结实率降低及形成双胚苗的细胞学基础。By using autotetraploid polyembryonic mutant rice IR36-Shuang as material, the process and characters of its double fertilization, embryo and endosperm development were observed with a laser scanning confocal microscopy. Some abnormalities including degradated ovary, abortive embryo sac, single fertilization, double-ovule and double-embryo were found during the double fertilization and embryo development in IR36-Shuang. The rate of the abnormalities was 46.67%, significantly higher than that in the control IR36-4x（33.00%）. Cytological and embryonic proofs were provided for seed setting decline and the initiation of additional embryo in the IR36-Shuang.

关 键 词：同源四倍体多胚苗水稻 双受精 生殖发育特征 发育生物草 激光扫描共聚焦显微术 

分 类 号：S511[农业科学—作物学]