水稻立枯丝核菌G蛋白β亚基基因的克隆、表达及序列分析  

Cloning,Expression and Characterization of G Protein β-subunit Gene in Rhizoctonia solani from Rice

作　　者：肖勇[1] 李双成[1] 初明光[1] 周鹏[1] 关鹏[1] 柳莉[1] 王玲霞[1] 郑爱萍[2] 李平[1] 

机构地区：[1]四川农业大学水稻研究所,四川温江611130 [2]四川省农业生物技术工程研究中心,四川温江611130

出　　处：《中国水稻科学》2008年第5期541-544,共4页Chinese Journal of Rice Science

基　　金：国家863计划资助项目(2006AA10A103);长江学者和创新团队发展计划资助项目(IRT0453)

摘　　要：根据Thanatephorus cucumeris G蛋白β亚基序列(AY884129)设计引物,对水稻立枯丝核菌AG-1IA的G蛋白β亚基基因进行了克隆。PCR结果得到1条约为1.9kb的扩增片段,包含1个约1.7kb的完整开放阅读框,编码366个氨基酸。同源性检索发现该序列与大量G蛋白β亚基基因明显同源,一致性介于57.34%～88.14%。根据其推导cDNA序列设计引物进行RT-PCR分析,发现该基因在对数生长期表达量最高,提示水稻立枯丝核菌AG-1IAG蛋白β亚基基因可能具有时空表达特性。A G protein β-subunit gene in Rhizoctonia solani AG-1IA of rice which causes rice sheath blight disease was isolated. A 1958-bp PCR fragment was obtained by using the specific primers designed from the conserved region in G protein β-subunit homologues from Thanatephorus cucumeris. Amino acid sequence analysis showed that the sequence involved 366 deduced amino acid, encoded a putative peptide which shared significant identity （57. 34%-- 88. 14%） with G protein β-subunit homologues from other species. The full-length of the PCR fragment was 1.9 kb with a 1.7-kb open reading frame of the G protein β-subunit gene in R. solani AG-1IA. The results of RT-PCR showed that the G protein β-subunit gene transcript was greatly expressed in all growth period especially at the exponential phase, which indicated that the expression of the G protein β-subunit gene in R. solani AG-1IA might be regulated temporally and spatially.

关 键 词：水稻 立枯丝核菌 G蛋白β亚基基因 克隆 基因表达 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]