艾灸促进胃黏膜细胞HSP70表达上调对细胞凋亡线粒体信号转导途径的影响  

Effect of moxibustion-induced up-regulation of HSP70 expression on mitochondrial signal transduction pathway during gastric mucosal cell apoptosis in rats

作　　者：易受乡[1] 郁洁[1] 常小荣[1] 吴伋用[1] 李振海[1] 唐森[1] 周国祥[1] 王小顺[1] 

机构地区：[1]湖南中医药大学针灸推拿学院,湖南省长沙市410007

出　　处：《世界华人消化杂志》2008年第24期2689-2694,共6页World Chinese Journal of Digestology

基　　金：国家自然科学基金资助项目;No.30772707~~

摘　　要：目的:探讨艾灸预处理抑制细胞凋亡、保护胃黏膜损伤的机制.方法:32只健康Wister大鼠随机分为4组,即捆绑组(A组)、模型组(B组)、艾灸穴位组(C组)和艾灸非穴组(D组),每组8只.艾灸穴位组和艾灸非穴组大鼠艾灸预处理8d,捆绑组和模型组大鼠只捆绑,不艾灸.除捆绑组外,其余各组采用无水乙醇灌胃法制备大鼠急性胃黏膜损伤模型.采用Western blot法检测大鼠胃黏膜细胞色素C(Cyt-C)的表达,免疫组织化学方法检测大鼠胃黏膜HSP70、胃黏膜细胞凋亡、凋亡活化因子1(Apaf-1)、Caspase-9和Caspase-3的表达.结果:与A组相比,B组大鼠胃黏膜HSP70表达,胃黏膜细胞凋亡指数(AI),胞质Cyt-C、Apaf-1、Caspase-9和Caspase-3含量明显增加(2.93±0.29vs2.51±0.22,3.52±0.77vs2.25±0.53,1.63±0.36vs0.75±0.23,4.32±0.67vs3.44±0.86,4.05±1.01vs3.76±0.82,3.55±0.86vs2.35±0.71,P<0.01或0.05).与B组相比,C组大鼠胃黏膜HSP70表达(3.93±0.36)明显增加(P<0.01),而细胞凋亡指数(1.53±0.45),胞质Cyt-C(0.97±0.26)、Apaf-1(2.24±0.49)、Caspase-9(2.43±0.73)、Caspase-3(1.97±0.61)均显著降低(P<0.01).与C组相比,D组大鼠胃黏膜HSP70表达(3.35±0.34)显著降低(P<0.01),而凋亡指数(3.06±0.81),Cyt-C(1.45±0.29),Apaf-1(3.16±0.66),Caspase-9(3.33±0.76),Caspase-3(2.98±0.86),显著升高(P<0.01或0.05).结论:艾灸通过上调大鼠胃黏膜细胞HSP70表达,继而作用于凋亡线粒体信号转导途径相关靶点,由此抑制胃黏膜细胞凋亡,达到保护胃黏膜损伤的作用,且这种保护作用具有一定的穴位特异性.AIM： To explore the mechanism of premoxibustion inhibiting cell apoptosis and protecting gastric mucosal damage. METHODS： Thirty-two healthy rats were randomly and averagely assigned into 4 groups, namely control group （group A）, model group （group B）, moxibustion groups （group C and D, pre-moxibustion at acupoints and non-acupoints respectively for 8 d）. The models of acute gastric mucosal damage were established by gastric injection of absolute ethyl alcohol in all the rats except those in group A. Western blot was used to detect the expression of cytochrome C （Cyt-C） while immunohistochemical method was used to detect the expression of heat shock protein 70 （HSP70）, apoptosis protease activator protein-1 （Apaf-1）, Caspase-9 and Caspase-3 as well as cell apoptosis in gastric mucosa. RESULTS： As compared with those in group A, the expression of HSP70, cell apoptosis index （AI）, Cyt-C in cytoplasm, Apaf-1, Caspase-9, and Caspase-3 contents were significantly increased in group B （2.93 ± 0.29 vs 2.51 ± 0.22; 3.52 ±0.77 vs 2.25 ± 0.53; 1.63 ± 0.36 vs 0.75 ± 0.23; 4.32 ± 0.67 vs 3.44 ± 0.86; 4.05 ± 1.01 vs 3.76 ± 0.82; 3.55 ± 0.86 vs 2.35 ± 0.71; P 〈 0.01 or 0.05）. In comparison with those in group B, the expression of HSP70 （3.93 ± 0.36） was significantly increased （P 〈 0.01）, but the AI （1.53 ± 0.45）, Cyt-C in cytoplasm （0.97 ± 0.26）, Apaf-1 （2.24 ± 0.49）, Caspase-9 （2.43 ± 0.73）, and Caspase-3 （1.97 ± 0.61） contents were significantly decreased in group C （all P 〈 0.01）. In compared with those in group C, the expression of HSP70 （3.35 ± 0.34） was significantly decreased （P 〈 0.01）, but the AI （3.06 ± 0.81）, Cyt-C in cytoplasm （1.45 ± 0.29）, Apaf-1 （3.16 ± 0.66）, Caspase-9 （3.33 ± 0.76）, and Caspase-3 （2.98 ± 0.86） contents were significantly increased in group D （P 〈 0.01 or 0.05）. CONCLUSION： Moxibustion may up-regulate the expression of HSP70 in gastric mucosa, and then act o

关 键 词：艾灸 HSP70 细胞凋亡 线粒体 细胞色素C CASPASE-9 Caspase-3 免疫组化 

分 类 号：R245[医药卫生—针灸推拿学]