黄芪等12味中药在体外培养中对人调节性T细胞分化的影响  

Effects of twelve Chinese herbs on human regulatory T cell differentiation in vitro

作　　者：董文毅[1] 胡刚正[2] 张博[1] 郑长青[2] 陈苏宁[1] 刘万里[1] 史业东[1] 

机构地区：[1]中国医科大学附属盛京医院中医科,辽宁省沈阳市110004 [2]中国医科大学附属盛京医院消化内科,辽宁省沈阳市110004

出　　处：《世界华人消化杂志》2008年第24期2770-2774,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨黄芪等12味中药对人调节性T细胞的分化的影响,为溃疡性结肠炎的中药组方研究提供实验依据.方法:分离正常人外周血淋巴细胞和单核细胞,在体外培养中用人肠道厌氧菌抗原刺激单核细胞,然后将激活的单核细胞与淋巴细胞混合培养,分别加入黄芪等12种不同的中药煎液,最后用流式细胞术检测其对CD4+CD25+FOXP3+调节性T细胞分化的影响.结果:与PBS组比较,CD4+细胞中CD25及FOXP3双阳细胞的百分比在黄芪及丹参组明显升高(5.6%±0.6%,5.5%±0.8%vs4.3%±0.8%;均P<0.01),同时CD4+CD25+细胞中FOXP3的表达率也显著升高(50.0%±3.8%,45.1%±3.3%vs30.9%±4.5%;均P<0.01).结论:黄芪和丹参可能通过调节性T淋巴细胞的分化平衡来发挥其治疗作用,可作为溃疡性结肠炎组方的首选中药.AIM： To investigate the effects of 12 Chinese herbals （including Astragalus） on the differentiation of human regulatory T cells, and to provide experimental basis for the treatment of ulcerative colitis （UC） using traditional Chinese medicine （TCM）. METHODS： Normal human peripheral lymphocytes and monocytes were seperated and cultured in vitro. The monocytes challenged by anaerobic intestinal bacterial antigens were cocultured with lymphocytes and then treated with 12 different decoctions of Chinese herbs including Astragalus. Flow cytometry was used to detect the differentiation of CD4^±CD25^±FOXP3^± regulatory T cells. RESULTS： As compared with that in the PBS control group, CD4^±CD25^±FOXP3^± regulatory T cell population increased significantly in the Astragalus-treated group and Salvia-treated group （5.6% ± 0.6%, 5.5% ± 0.8% vs 4.3% ± 0.8%; both P 〈 0.01）, and FOXP3^± expression in CD4^±CD25^± cells increased significantly as well （50.0% ± 3.8%, 45.1% ± 3.3% vs 30.9% ± 4.5%; both P 〈 0.01）. CONCLUSION： Salvia Miltiorrhiza and Astragalus may be the first choice for the treatment of ulcerative colitis due to their regulatory effects on the balance of T lymphocyte differentiation.

关 键 词：溃疡性结肠炎 炎症性肠病 T淋巴细胞 黄芪 丹参 中药 

分 类 号：R285.5[医药卫生—中药学]