hIGF-1基因治疗老龄大鼠勃起功能障碍的剂量及时效研究  

Dose and Long-Term Effect of hIGF-1 Injection for Erectile Dysfunction in Aged Rats

作　　者：蒲小勇[1] 王行环[2] 王怀鹏[1] 高炜城[1] 杨中华[1] 李世林[1] 邓春华[3] 毛向明[4] 

机构地区：[1]广东省人民医院泌尿外科,广东广州510080 [2]武汉大学中南医院泌尿外科,湖北武汉430071 [3]中山大学第一附属医院泌尿外科,广东广州510080 [4]南方医科大学附属南方医院泌尿外科,广东广州510515

出　　处：《中华男科学杂志》2008年第8期690-694,共5页National Journal of Andrology

基　　金：中国博士后科学基金(20060400779);广东省自然科学基金(07001203);广东省医学科研基金(A2008033)

摘　　要：目的:探讨阴茎海绵体内注射胰岛素样生长因子1(hIGF-1)基因提高老年性大鼠阴茎勃起功能的最佳剂量和作用实效。方法:4月龄SD雄性大鼠10只(青年组);24月龄SD雄性大鼠40只,随机分为5组,每组10只:PBS对照组、hIGF-1基因10μg注射组、100μg注射组和1000μg注射组,注射后在不同时间段4周和8周行电刺激检测大鼠阴茎海绵体内压(ICP)和平均动脉压(MAP)的测定,分析比较不同剂量和不同时间hIGF-1基因治疗的效果;RT-PCR检测hIGF-1基因mRNA的表达。结果:电刺激发现老年组较青年组ICP/MAP和总ICP明显降低(P<0.05)。4和8周的治疗之后,10μg注射组、100μg注射组和1000μg注射组较PBS对照组ICP/MAP和总ICP均明显提高(P<0.05);100μg注射组和1000μg注射组在4和8周时ICP/MAP和总ICP值和青年组相仿;10μg注射组虽然不能达到和青年组相仿的治疗效果,但仍然有治疗意义;所有hIGF-1基因治疗组均可检测到hIGF-1 mRNA的表达。结论:hIGF-1基因治疗能够提高老龄大鼠的勃起功能,100μg即可达到有效的治疗,hIGF-1基因治疗至少能够维持8周时间。Objective： To investigate the best dose and the long-term effect of the human insulin-like growth factor-1 （ hIGF-1 ） gene injection into the penis of aged rats. Methods： Included in this study were 10 young （4 months old） and 40 aged （24 months old） Sprague-Dawley male rats, the latter equally divided into a PBS control and a 10 μg, a 100 μg and a 1 000 μg hIGF-1 injection group.Electrical stimulation was conducted 4 and 8 weeks after hIGF-1 injection into the penile corpus cavernous of the rats to detect the intracavernous pressure （ICP） and mean arterial pressure （MAP）. Dose - and time - associated therapeutic results were analyzed and the mRNA expression of hIGF-1 determined by RT - PCR. Results： ICP, MAP and total ICP were significant decreased by electrical stimulation in the aged rats as compared with the young ones （P 〈 0.05） , statistically increased in the three hIGF-1 dose groups in comparison with the PBS controls （P 〈 0.05） , and showed no obvious difference between the young rats and the latter two dose groups at 4 and 8 weeks. Although less obvious effect was achieved in the 10 μg group than in the young rats, the therapeutic result was still of significance. The mRNA expression of the hIGF-1 gene was confirmed in all the hIGF-1 treated rats. Conclusion ： The hIGF-1 therapy can improve erectile function in aged rats, 100 μg suffices for effective erection and the effect may last at least 8 weeks for a single dose.

关 键 词：勃起功能障碍 老龄 胰岛素样生长因子1 基因治疗 大鼠 

分 类 号：R698.1[医药卫生—泌尿科学]