抑瘤饮增加小鼠S180移植瘤巨噬细胞浸润及TNF-α和iNOS表达  

Chinese medicine compound "YILIUYIN" enhances the macrophage infiltration and the expression of TNF-α and iNOS in transplanted S180 tumor of mouse

作　　者：李铁民[1] 王润田[1] 韩志鹏[1] 杨志强[1] 崔 丁军颖[1] 邓郁青[1] 王平[1] 张征峥[1] 

机构地区：[1]河北医科大学基础所免疫室,石家庄050017

出　　处：《免疫学杂志》2008年第5期559-562,567,共5页Immunological Journal

基　　金：河北省中医药管理局资助项目(2006054)

摘　　要：目的动态研究中药复方抑瘤饮对小鼠S180移植瘤内巨噬细胞(Mφ)浸润及TNF-α和iNOS表达的影响,揭示其体内抗瘤免疫机制。方法Balb/c小鼠右腋皮下接种S180肿瘤细胞,24h后,抑瘤饮组小鼠每日灌服抑瘤饮,对照组小鼠每日灌服等量凉开水。分别于第10、20、30天和第40天杀鼠取瘤制成切片,免疫组化染色法检测肿瘤组织内Mφ浸润及TNF-α和iNOS表达,以图像分析系统对染色结果进行测定。结果所有4个时间点抑瘤饮组Mφ浸润均显著高于对照组;第10天时抑瘤饮组TNF-α和iNOS表达与对照组无差异,第20、30天和第40天时高于对照组。结论抑瘤饮体内抗瘤作用可能与增加肿瘤组织中Mφ浸润及TNF-α和iNOS表达有关。Objective To study the dynamic effects of Chinese compound medicine YILIUYIN on the macrophage infiltration and the expressions of TNF-α and iNOS in transplanted S180 tumor of mouse and to reveal the anti-tumor immune mechanism of YILIUYIN in vivo, Methods Balb/c mice were inoculated with S180 tumor cells subcutaneously in the right axilla, and 24 hours later, the mice were divided into YILIUYIN group and control group, which were orally and daily administrated with YILIUYIN and the equal volume of cold boiled-water, respectively. The mice were sacrificed and the tumors were acquired at day 10, day 20, day 30, and day 40, respectively. The macrophage infiltration and the expressions of TNF-α and iNOS were detected by immunohistochemistry staining of tumor sections. Results The macrophage infiltration of YILIUYIN group was significantly higher than that of control group at all the four time-points; the expressions of TNF-α and iNOS of YILIUYIN group had no differences with control group at time-point of day 10, but was higher than that of control group at time-points of day 20, day 30, and day 40. Conclusion The anti-tumor effects of YILIUYIN in vivo might correlate with up-regulating of the macrophage infiltration and the expressions of TNF-α and iNOS in tumor tissue.

关 键 词：抑瘤饮 S180 巨噬细胞 肿瘤坏死因子-α 诱导型一氧化氮合酶 

分 类 号：R285.5[医药卫生—中药学]