不同材质微芯片内细胞生长状态分析  

CELL VIABILITY STUDY IN MICROCHIP OF DIFFERENT MATERIALS

作　　者：邵建波[1] 吴蕾[1] 赵辉[1] 郑允焕[1] 吴建璋[1] 金庆辉[1] 赵建龙[1] 

机构地区：[1]中国科学院上海微系统与信息技术研究所

出　　处：《生物物理学报》2008年第4期268-272,共5页Acta Biophysica Sinica

基　　金：国家自然科学基金项目(30771115);上海市科委项目(0652nm016;06JC14081;0752nm021;06xd14037);973项目(2005CB724305)~~

摘　　要：微系统技术在细胞生物学方面的研究中已得到广泛应用。了解细胞在微系统芯片内的生长状态,对于利用微系统技术进行细胞研究有重要的指导意义。玻璃和聚二甲基硅氧烷(polydimethylsiloxane,PDMS)是目前制作细胞培养微芯片的主要材料。通过向以二者为基底材料制作的细胞培养微芯片内导入内皮细胞进行培养,利用实验室构建的细胞成像分析系统观察和分析细胞在不同基底材料的芯片内5天的增殖情况,同时研究了基底材料的预处理方法以及培养基对细胞增殖的影响。Microsystem technologies have been widely applied in cell biology study. Understanding the viability of cells in microsystem is important to direct the cell research by the way. Glass and polydimethylsiloxane are familiar in the fabrications of microchip for cell research. They were used to bond with PDMS to make cell culture chip for cell viability study. The endothelial cells were infused in the microchip and their growth in five days were observed, and the influences of the treatment of chips before cell infused and the changes of culture medium were studied.

关 键 词：细胞培养 玻璃 聚二甲基硅氧烷 微芯片 增殖速率 

分 类 号：Q813.1[生物学—生物工程]