石刁柏体细胞胚发生过程中多胺代谢酶和过氧化氢清除酶的活性变化  

Changes in Activities of Enzymes Involved in Polyamines Metabolism and Elimination of Hydrogen Peroxide during Somatic Embryogenesis in Asparagus officinalis L.

作　　者：邓白平[1] 陈小飞[2] 鲁旭东[2] 刘华英[3] 萧浪涛[2] 

机构地区：[1]湖南农业大学资产管理处,长沙410128 [2]湖南农业大学湖南省植物激素与生长发育重点实验室,长沙410128 [3]广西师范大学生命科学技术学院,广西桂林541001

出　　处：《植物生理学通讯》2008年第4期647-650,共4页Plant Physiology Communications

基　　金：国家自然科学基金(30540019);湖南农业大学2007年度教学改革研究项目

摘　　要：石刁柏体细胞胚发生过程中精氨酸脱羧酶(ADC)活性变化趋势与内源腐胺的含量变化相同,多胺氧化酶(PAO)活性变化幅度较小。外源腐胺对ADC活性的影响与不作外源腐胺处理的差异不显著性,但促进体细胞胚发生前期的PAO活性,过氧化物酶和过氧化氢酶活性受外源腐胺的影响很小,两者的活性变化与PAO活性变化趋势一致。In order to get a better understanding on how the polyamines play roles in somatic embryogenesis, the changes in enzymes activities involved in polyamines metabolism and hydrogen peroxide eliminating during somatic embryogenesis in Asparagus officinalis were investigated. The arginine decarboxylase （ADC） had the same change trend as endogenous putrescine during the initial progress. The polyamine oxidase （PAO） activity varied in a gentle way. The ADC activity was not affected significantly by exogenous putrescine applied during the later progress of somatic embryogenesis. While the PAO was up-regulated in the early stage of somatic embryogenesis, the activities of catalase and peroxidase, which varied as same as the PAO activity, were little affected by exogenous putrescine.

关 键 词：体细胞胚胎发生 多胺代谢酶 过氧化氢清除酶 

分 类 号：Q946[生物学—植物学]