X、Y精子分离纯度评价方法的研究进展  

Progress on methods for purity assessment of separated chromosome X-or Y-bearing sperm

作　　者：郭家明[1] 朱化彬[1] 王栋[1] 张林波[2] 郝海生[1] 杜卫华[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100094 [2]吉林农业大学生命科学学院,长春130118

出　　处：《遗传》2008年第9期1115-1120,共6页Hereditas（Beijing)

基　　金：国家自然科学基金资助项目(编号:30671502);国家高技术研究发展规划(863项目)项目(编号:2006AA10Z143)资助~~

摘　　要：对检测分析分离精液中X、Y精子纯度的方法进行了综述,并将各种方法的原理、技术操作过程和方法的优缺点进行了比较分析。认为如能在技术上有所突破,提高方法的灵敏度、精确性,降低检测时间,单精子巢式PCR方法将可能成为一种低成本、常规化的检测手段,在精子分离方法优化研究中发挥更大的作用,并推动其他单精子遗传检测技术取得新进展。In this review of methods for purity assessment of isolated chromosome X- and Y-beating sperm, we compared the principles, operating procedures, as well as pros and cons for various methods. We conclude that nested PCR of single sperm will become a conventional and popular method with lower costs, and the method will play a very important role in optimizing the X, Y sorting method, if the sensitivity and accuracy of the method can be increased and the testing time decreased, and promote the new progress in other genetic testing techniques on single sperm.

关 键 词：精子 分离精子 纯度评价 单精子巢式PCR 

分 类 号：Q813[生物学—生物工程]