二氧化硫对低氧性肺动脉高压大鼠肺动脉结构的影响  

Effect of sulfur dioxide on pulmonary vascular structure of hypoxic pulmonary hypertensive rats

作　　者：田悦[1] 汤秀英[2] 金红芳[1] 唐朝枢[3] 杜军保[1] 

机构地区：[1]北京大学第一医院儿科,100034 [2]北京大学第一医院电镜室,100034 [3]北京大学医学部生理与病理生理学系

出　　处：《中华儿科杂志》2008年第9期675-679,共5页Chinese Journal of Pediatrics

基　　金：国家杰出青年科学基金资助项目（30425010）;国家自然科学基金资助项目（30571971）;国家自然科学基金重点资助项目（30630031）;国家重点基础研究发展规划资助项目（2006CB503800）;国家长江学者奖励计划

摘　　要：目的探讨二氧化硫（sulfur dioxide，SO2）对于低氧性肺动脉高压大鼠肺动脉结构的影响。方法将大鼠分为3组：对照组（n=8）、低氧组（n=8）和低氧＋SO2组（n=10，给予Na2SO3／NaHSO3）。对低氧组和低氧＋SO2组大鼠进行低氧处理21 d。同时对照组置于常氧环境。检测各组大鼠肺动脉平均压，并通过光镜检测肌型小动脉相对中膜厚度（RMT），应用透射电镜观察各组大鼠肺小动脉超微结构的变化，检测血浆SO2含量。结果低氧组大鼠肺动脉平均压[（5．12±0．51）kPa]较对照组[（2．25±0．50）kPa]高（t=5．091，P〈0．01），低氧组肺动脉RMT测定值（9．66±1．27）较对照组（6．83±1．57）高（t=3．392，P〈0．01），超微结构观察显示低氧组大鼠肺小动脉内皮细胞体积增大变性，内弹力层疏松厚薄不均。平滑肌细胞体积增大，细胞器丰富，细胞间见胶原原纤维增多，同时低氧组血浆SO2含量[（27．01±4．17）umol／L]较对照组[（33．36±5．62）umol／L]低（t=3．767，P〈0．05）；低氧＋SO2组大鼠的肺动脉平均压[（3．94±0．33）kPa]较低氧组[（5．12±0．51）kPa]低（t=2．712，P〈0．01），低氧＋SO2组RMT测定值（6．97±1．83）较低氧组（9．66±1．27）低（t=3．009，P〈0．01），超微结构观察显示给予SO2干预后上述改变较低氧组明显改善，同时血浆SO2含量[（29．89±4．52）umol／L]较低氧组[（27．01±4．17）~mol／L]高（t=1．263，P〉0．05）。结论SO2对低氧性肺动脉高压大鼠肺小动脉结构具有重要的调节作用。Objective Hypoxic pulmonary hypertension is an important pathophysiologic process of various cardiovascular diseases. Sulfur dioxide （ SO2 ） was considered as a kind of toxic gas previously, but recent studies suggested that SO2 could act as a key bioactive molecule in the pathogenesis of cardiovascular diseases. Therefore, this study was designed to examine the effect of sulfur dioxide on pulmonary vascular structure of hypoxic pulmonary hypertensive rats treated with SO2 donor substances. Methods The rats were randomly divided into 3 groups ： control group （ n = 8 ） , hypoxic group （ n = 8 ） and hypoxic ＋ SO2 group （ n -10,treated with SO2 donor Na2SO3/NaHSO3 ）. The rats of hypoxic group and hypoxic ＋ SO2 group were under a hypoxic condition for 21 days,while the rats of control group were exposed to room air. The mean pulmonary artery pressure was tested by means of right cardiac catheterization and the content of SO2 in plasma was investigated by high performance liquid chromatography （HPLC）. The change in relative medial thickness （RMT） of pulmonary arteries was examined under optical microscope. The ultra-structural changes were observed under a transmission electron microscope. The data were analyzed through one-way analysis of variance （ANOVA） by SPSS 13. 0 software. Results Compared with control group [ （ 2. 25± 0. 50 ） kPa ] , the mean pulmonary artery pressure of hypoxic group [ （ 5.12 ± 0.51 ） kPa ] raised significantly （ t = 5.091, P〈0.01） and RMT of hypoxic group （9.66 ± 1.27） compared with control group （6.83 ± 1.57） significantly raised （ t = 3. 392, P 〈 0.01 ） . Ultrastructural observation showed the proliferation and degeneration of endothelial cells in small pulmonary arteries in rats with pulmonary hypertension. The internal elastic lamina was irregular. The proliferation of medial smooth muscle cells of arteries was shown at the level of respiratory bronchioles. The collagens also increased. Meanwhile, compared with control gro

关 键 词：高血压 肺性 二氧化硫 大鼠 

分 类 号：R686[医药卫生—骨科学]