血小板活化因子受体拮抗剂对内毒素血症幼年大鼠肠道黏液蛋白损伤的作用  

Effects of platelet activating factor receptor antagonist in intestinal mucin-2 during endotoxemia on young rats

作　　者：王丽杰[1] 孙梅[1] 

机构地区：[1]中国医科大学附属盛京医院儿科,沈阳110004

出　　处：《中华儿科杂志》2008年第9期680-683,共4页Chinese Journal of Pediatrics

摘　　要：目的探讨血小板活化因子（PAF）受体拮抗剂在内毒素血症幼年大鼠肠道黏液蛋白（MUC2）损伤中的作用。方法18日龄Wistar大鼠，随机分为对照组和实验组，实验组又分为内毒素组（LPS组）、PAF受体拮抗剂预防组（简称预防组）和PAF受体拮抗剂治疗组（简称治疗组），每一时点（注射LPS后1．5、3、6、24、48、72h）各8只，注射LPS后1．5、3、6、24、48、72h取回肠。LPS组和对照组分别腹腔注射内毒素5mg／kg及生理盐水1ml／kg；预防组于注射LPS前30min、治疗组于注射LPS后30min腹腔注射PAF受体拮抗剂BN52021 5mg／kg。用透射电镜做形态学检查。应用免疫组化方法检测肠黏膜中MUC2变化。结果电镜下对照组肠微绒毛及细胞问紧密连接未见异常。LPS组上皮细胞连接增宽；微绒毛变细、稀疏，部分断裂、脱落；细胞器受损。预防组和治疗组改变较LPS组轻。对照组MUC2蛋白均匀地分布于回肠上皮细胞表面，MUC2蛋白染色阳性的杯状细胞轻度膨胀、扩张。实验组细胞表面MUC2阳性染色明显减少，分布不均。对照组MUC2表达平稳且水平较高，其他3组均下降，LPS组降低最为明显，低谷在注射后6h（0．1841±0．0047），明显低于对照组（0．2091±0．0060）（P〈0．01），预防组及治疗组变化趋势同LPS组，各时点MUC2均较LPS组高，方差分析提示组内及组间比较差异有统计学意义（P〈0．05）。结论PAF在内毒素血症肠道黏液屏障功能损伤中可能起一定作用，预防和治疗性应用PAF受体拮抗剂BN52021可减轻肠损伤。Objective This study aimed to investigate the roles of platelet activating factor （PAF） receptor antagonist in damage to intestinal mucin-2 （MUC2） during endotoxemia on young rats. Methods Eighteen-day-old Wistar rats were randomized to be treated with lipopolysaccharide （LPS） （5 mg/kg ） ,LPS plus PAF receptor antagonist and normal saline injection （ control ）. PAF receptor antagonist BN52021 5 mg/kg was administered 30 minutes before or after LPS injection （pretreatment group or treatment group）. The ileum specimens （n = 8 ） were harvested at 1.5,3,6,24,48 and 72 hours after LPS injection. Transmission electron microscopy was used for morphologic evaluation. Immunohistochemistry was used to determine MUC2 in intestinal mucosa. Results Microvilli and tight junctions were intact in the control group. Enlargement of tight junctions were seen in the LPS group and microvilli were thin, rare or disrupted, shed. The rough endoplasmic reticulum, mitochondria, and glycogen particles were injured. The changes of the pretreatment and treatment group were slightly milder than that in the LPS group. Ileum of a control rat in which a thin layer of mucus covering the epithelial surface and mucin-containing goblet cells appeared very distended. The experiment group showed a decrease or irregularly distributed membranous mucus expression. The MUC2 content of absorbance significantly decreased in the LPS challenged group compared with that in the control group （P 〈0. 01 ）, and reached a nadir at 6 hours （0. 1841 ±0. 0047） vs. the control group （0. 2091 ±0. 0060） （P 〈0. 01 ）. The tendency of the level of MUC2 in the pretreatment and treatment group was the same as that of the LPS group, and the level of MUC2 in the pretreatment and treatment group was higher than that in the LPS group at each time point. ANOVA analysis showed that the inter-group and intra-group difference had statistical significance （ P 〈 0. 05 ）. Conclusions PAF may play some roles in the injury of intesti

关 键 词：血小板活化因子 黏液 胃黏蛋白 肠黏膜 

分 类 号：R686[医药卫生—骨科学]