通心络血清对U937单核巨噬细胞MMP-9及TIMP-1表达与分泌的影响  

Effect of the sera of rabbits fed with Tongxinluo on MMP-9 and TIMP-1 expression and secretion in U937 monocyte-derived macrophages

作　　者：刘金来[1] 朱强锋[1] 郝宝顺[1] 

机构地区：[1]中山大学附属第三医院心内科,广东广州510630

出　　处：《南方医科大学学报》2008年第9期1703-1706,共4页Journal of Southern Medical University

摘　　要：目的探讨通心络含药血清对U937单核细胞源巨噬细胞基质金属蛋白酶-9及金属蛋白酶组织抑制因子-1表达与分泌的影响。方法通过喂养高脂饲料,建立兔动脉粥样硬化模型后,予通心络药液及辛伐他汀药液灌胃,获得含药血清。分别予不同浓度含药血清培养U937单核细胞源巨噬细胞24 h,应用逆转录聚合酶链式反应检测基质金属蛋白酶-9及金属蛋白酶组织抑制因子-1 mRNA的表达,以及应用酶联免疫吸附试验检测基质金属蛋白酶-9及金属蛋白酶组织抑制因子-1蛋白的分泌。结果通心络含药血清呈剂量依赖性抑制U937单核细胞源巨噬细胞基质金属蛋白酶-9mRNA的表达与分泌,而对金属蛋白酶组织抑制因子-1 mRNA的表达与分泌未见影响。结论通心络可能通过抑制单核巨噬细胞基质金属蛋白酶-9的表达与分泌,而起到稳定斑块的作用。Objective To investigate the effect of the sera of rabbits fed with Tongxinluo on the expression and secretion of matrix metalloproteinase-9 （MMP-9） and tissue inhibitor of metalloproteinase-1 （TIMP-1） in U937 monocyte-derived macrophages. Methods Atherosclerosis was induced in rabbits by high-cholesterol feeding, and the serum was obtained from the rabbits after administration of the aqueous solution of Tongxinluo or simvastatin by gavage. U937 monocyte-derived macrophages were incubated with the sera at different concentrations for 24 hours, and the changes in MMP-9 and TIMP-1 gene expression and secretion were detected by RT-PCR and enzyme-linked immunosorbent assay, respectively. Results The serum of rabbits fed with Tongxinluo concentration-dependently inhibited the expression and secretion of MMP-9 in U937 macrophages, but did not affect TIMP-1 expression or secretion. Conclusion Tongxinluo may stabilize the atherosclerotic plaques by inhibiting the expression and secretion of MMP-9.

关 键 词：通心络 巨噬细胞 基质金属蛋白酶-9 金属蛋白酶组织抑制因子-1 

分 类 号：R285[医药卫生—中药学]