数种5-羟色胺受体拮抗剂对日本血吸虫运动性的影响  

THE EFFECTS OF SEVERAL 5HT RECEPTOR ANTAGONISTS ON THE MOTOR ACTIVITY IN SCHISTOSOMA JAPONICUM

作　　者：王丽平[1] 史晓丽[1] 沈丽琳[1] 

机构地区：[1]南京农业大学动物医学院

出　　处：《南京农业大学学报》1997年第4期83-87,共5页Journal of Nanjing Agricultural University

摘　　要：采用肉眼观察法测定不同５－羟色胺（５－ＨＴ）受体拮抗剂对日本血吸虫运动性的影响。结果表明：（１）１０－３ｍｏｌ／Ｌ和１０－４ｍｏｌ／Ｌ的５－ＨＴ作用虫体３０ｍｉｎ后，虫体运动显著增强，而１０－５ｍｏｌ／Ｌ时，虫体运动稍有增强。１０－３ｍｏｌ／Ｌ色胺（ｔｒｙｐｔａｍｉｎｅ）也可显著增强虫体运动，但１０－４ｍｏｌ／Ｌ、１０－５ｍｏｌ／Ｌ时，作用则不显著。（２）用１０－３ｍｏｌ／Ｌ赛庚啶（ｃｙｐｒｏｈｅｐｔａｄｉｎｅ）、曲拉唑酮（ｔｒａｚｏｄｏｎｅ）、米安舍林（ｍｉａｎｓｅｒｉｎ）、螺环哌啶酮（ｓｐｉｒｏｐｅｒｉｄｏｌ）、氟哌丁苯（ｈａｌｏｐｅｒｉｄｏｌ）作用虫体１ｈ后，除ｈａｌｏｐｅｒｉｄｏｌ外，其余均可对虫体产生不同程度的拮抗作用。（３）先用１０－４ｍｏｌ／Ｌ５－ＨＴ培养虫体３０ｍｉｎ，当虫体运动增强后，再用上述拮抗剂作用虫体１ｈ，发现ｃｙｐｒｏｈｅｐｔａｄｉｎｅ、ｍｉａｎｓｅｒｉｎ、ｔｒａｚｏｄｏｎｅ对虫体有拮抗作用，ｓｐｉｒｏｐｅｒｉｄｏｌ略有拮抗，而ｈａｌｏｐｅｒｉｄｏｌ有一定协同兴奋作用，这些拮抗剂不仅对内源性５－ＨＴ有作用，而且对外源性５－ＨＴ均有拮抗性。初步判定，日本血吸虫体内存在５－ＨＴ受体。Motor activity was determined with drugs at different concentration under a microscope. The results showed that S. japonicums were obviously stimulated by 5hydroxytryptamine (10 -3 , 10 -4 mol/L) and tryptamine (10 -3 mol/L). Several vertebrate 5hydroxytryptamine antagonists, cyproheptadine, mianserin and trazodone, at concentration around 10 -3 mol/L not only blocked the effects of endogenous 5HT but also reduced exogenous 5HTinduced increases in the motor activity of S. japonicum . Spiroperidol, the other 5HT antagonist, had no influence on the motility. To our interest, haloperidol had coordinated excitation all along. The above effects preliminarily suggested the presence of 5HT receptor in S. japonicum and it′s difference from mammalian′s.

关 键 词：日本血吸虫 拮抗剂 5-羟色胺 受体 运动性 

分 类 号：S852.735[农业科学—基础兽医学]