大豆花叶病毒(SMV)的侵染对大豆叶片过氧化物酶及其同功酶的影响  

EFFECTS OF SMVINFECTION ON PEROXIDASE AND ITS ISOZYMES OF SOYBEAN LEAVES

作　　者：沈文飚[1] 徐朗莱[1] 胡蕴珠[1] 智海剑 孔军礼 

机构地区：[1]南京农业大学理学院

出　　处：《南京农业大学学报》1997年第4期88-92,共5页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金

摘　　要：研究了对ＳＭＶ抗性不同的３个大豆品种在接种ＳＭＶ－ａ株系后叶片过氧化物酶活性及其同功酶的动态变化。结果表明，侵染的早期（接种后３ｄ），抗病品种科丰１号的过氧化物酶活性急剧上升，出现了新的ＰＩ为４．４０和９．７３的同功酶；感病品种徐豆１号和１１３８－２的变化则相对较小。侵染的中后期（接种后６ｄ和９ｄ），感病品种的过氧化物酶活性迅速上升，且徐豆１号也出现了ＰＩ为４．４０和９．７３的同功酶；抗病品种过氧化物酶活性略有下降，ＰＩ为４．４０和９．７３的同功酶消失。ＳＭＶ侵染早期，抗病品种过氧化物酶活性的急剧增加以及ＰＩ为４．４０和９．７３的同功酶的出现可能与抗病性密切相关。Dynamic changes in activity and electrophoretogram of peroxidase were studied with leaves of resistant and susceptible soybean cultivars during infection by SMVa. The results suggested that peroxidase activities of resistant soybean cultivar (Kefeng1) increased rapidly, and two new isozymes of PI 4.40 and 9.73 appeared at the early stage of infection (three days after inoculation). On the other hand, the changes of peroxidase were relatively smaller in leaves of susceptible soybean cultivars (Xudou1 and 11382). At the middle and late stages of infection (six days and nine days after inoculation), peroxidase activities showed a greater increase in susceptible cultivars, and two identical isozymes of PI 4.40 and 9.73 in Xudou1 were found. While peroxidase activities of resistant soybean cultivar decreased slightly and the two new isozymes disappeared. In a word, rapid increment of peroxidase activities and appearance of two isozymes with PI 4.40 and 9.73 at the early stage of infection might be positively correlated with disease resistance to SMV in resistant cultivars.

关 键 词：大豆 花叶病毒 过氧化物酶 同功酶 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]