VEGF反义寡核苷酸对裸鼠膀胱癌移植瘤的抑制作用研究  

Inhibition of VEGF antisense oligonucleotides on human bladder carcinoma in nude mice

作　　者：高渝[1] 张唯力[1] 曾翔[1] 王志刚[2] 冉海涛[2] 李攀[2] 

机构地区：[1]重庆医科大学附属第二医院泌尿外科 [2]重庆医科大学医学影像学研究所,重庆400010

出　　处：《重庆医科大学学报》2008年第9期1084-1087,共4页Journal of Chongqing Medical University

摘　　要：目的:观察以脂质体为载体介导血管内皮生长因子(Vascular endothelial growth factor,VEGF)反义寡核苷酸(Antisense oligonucleotides,ASODN)转染对裸鼠膀胱癌移植瘤的抑制作用。方法:建立人膀胱癌裸鼠皮下移植瘤模型,以脂质体为载体介导VEGFASODN进行转染。观察裸鼠移植瘤生长情况,免疫组化SP法测Ki67、CD34、VEGF的表达,计算微血管密度、细胞增殖指数,TUNEL法测细胞凋亡指数。结果:反义组与正义组的抑瘤率分别为71.08%和7.19%,反义组VEGF的表达、微血管密度、细胞增殖和凋亡指数与正义组及对照组相比均有明显差异,正义组与对照组之间各检测指标差异无显著性。结论:VEGF反义寡核苷酸能够抑制人膀胱癌裸鼠皮下移植瘤的生长和微血管的生成,可能成为日后膀胱癌基因治疗的重要方向。Objective：To study the inhibiting effect of VEGF antisense oligonucleotides carried by liposome on the growth of human bladder carcinoma in nude mice. Methods：The nude mice model of human bladder carcinoma was established and VEGF antisense oligonucleetides were carried by liposome to be transfected.The cell proliferation index,microvessel density （MVD） and the expression of VEGF were detected with immunohistochmistry streptavidin-biotin peroxidase（SP） method and the cell apoptosis index was determined with Tunnel technology. Results：The growth inhibitory rate in antisense experimental group and sense experimental group were 71.08% and 7.19% respectively. The differences in the cell proliferation index,apoptosis index, microvessel density（ MVD ） and the expression of VEGF between the antisense experimental group and the sense experimental group or the control group had significances. There was no statistical significance in the detection indexes between the sense experimental group and the control group. Conclusions：Tbe VEGF antisense oligonucleotides can inhibit the neevascularization and the growth of bladder carcinoma in nude mice,which might be an important gene therapeutic strategy for the bladder carcinoma in future.

关 键 词：血管内皮生长因子 反义寡核苷酸 膀胱癌 

分 类 号：R737.14[医药卫生—肿瘤]