玻璃体腔注射曲安奈德治疗兔视网膜水肿的实验研究  

Experimental study on intravitreous injection with domestic triamcinolone acetonide for retinal edema in rabbits

作　　者：胡晓鹏[1] 倪寿翔[1] 李立[1] 周希瑗[1] 

机构地区：[1]重庆医科大学附属第二医院眼科,重庆400010

出　　处：《重庆医科大学学报》2008年第9期1088-1091,1107,共5页Journal of Chongqing Medical University

基　　金：重庆医科大学启动基金(0200100313)

摘　　要：目的:探讨常规剂量国产曲安奈德(Triamcinolone acetonide,TA)玻璃体腔注射治疗兔视网膜水肿的作用机制。方法:将健康新西兰大白兔48只随机分为A、B两组,均对右眼采用氩激光直接光凝法封闭视网膜静脉建立视网膜水肿的模型。A组24只兔右眼不做任何处理作为对照组,B组24只兔右眼在建模后1d玻璃体腔注射TA4mg/0.1ml作为实验组,A组和B组分别于注药后3d、1周、2周及1月各时间点随机处死6只动物,光镜下观察视网膜各层结构变化,利用原位杂交法检测各组兔视网膜组织中VEGF mRNA的表达,免疫组化法检测IL-6的表达情况。结果:48只兔均成功建立兔视网膜水肿模型,光镜下TA实验组视网膜组织形态改变优于对照组,实验组兔视网膜组织中VEGF mRNA以及IL-6的表达明显低于对照组,差异有显著性意义(P<0.05)。结论:TA能够有效降低视网膜水肿后VEGF及IL-6在视网膜组织中的表达,减轻视网膜的水肿,保护视网膜。Objective：To investigate the mechanism of intravitreous injection with conventional dosage of domestic triamcinolone acetonide （TA）for retinal edema in rabbits. Methods：The model of retinal edema was established with argon laser photocoagulation to retinal vein in fourty-eight healthy purebred New Zealand white rabbits. The right eye of A group wasn＇t injected anything as the control group, and the right eye of B group was injected with TA suspension 4mg（0.1ml）as the TA group. Six animals were killed randomly 3 days, 1 week, 2 weeks, and 1 month after the injected. The changes of the retinal edema were observed by light microscopy. The expression of VEGF mRNA and IL-6 were observed respectively by hybridization in situ and immunohistochemical method. Results：The model of retinal edema was established in all the eyes. The pathologic changes in TA group were better than that in control group. The expressions of VEGF mRNA and IL-6 in control group were higher than in TA group （P〈0.05）. Conclusion： Intravitreous injection of domestic TA may low the expression of VEGF mRNA and IL-6 in the model of retinal edema effectively and protect the retina.

关 键 词：曲安奈德 视网膜静脉阻塞 药物治疗 

分 类 号：R969.4[医药卫生—药理学]