穿心莲对肝硬化大鼠肠道细菌过度生长、细菌移位和肠道通透性的作用  

Effect of andrographitis on intestinal bacterial overgrowth,bacterial translocation and intestinal permeability in cirrhotic rats

作　　者：邢铭友[1] 吴春明[2] 覃慧敏[3] 李洪涛[1] 宋建新[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院感染科,湖北武汉430030 [2]温州市中医院肝病科,浙江温州325000 [3]宜昌市中心人民医院,湖北宜昌443003

出　　处：《中国医院药学杂志》2008年第13期1064-1067,共4页Chinese Journal of Hospital Pharmacy

基　　金：湖北省卫生厅科研基金(编号:NX200506)

摘　　要：目的:探讨穿心莲对肝硬化大鼠肠道细菌过度生长、细菌移位和肠道通透性的作用,为其临床应用提供依据。方法:建立大鼠四氯化碳肝硬化模型。将30只肝硬化大鼠随机分为4组:AN组(n=8,穿心莲0.5g·kg-1·d-1)、NF组(n=8,诺氟沙星0.3g·kg-1·d-1)、AN+NF组(n=8,穿心莲0.5g·kg-1·d-1+诺氟沙星0.3g·kg-1·d-1)、CN组(n=6,生理盐水4mL·kg-1·d-1),给药2周。取外周静脉血检测血浆ALT、AST、TP、TNF-α、NO水平,并获取肠系膜淋巴结、血液、肝、脾和回肠内容物做细菌培养。采用高压液相色谱法间接检测肠黏膜通透性,部分肝脏和回肠组织作病理切片。结果:与CN组相比,AN组、NF组和AN+NF组的NO、TNF-α的浓度显著降低(P<0.05,P<0.01),回肠细菌计数和细菌移位发生率显著下降(P<0.01)。AN组和NF组的肠道通透性显著下降(P<0.05)。病理检查发现CN组肝硬化大鼠回肠绒毛变短、破坏,炎症细胞浸润增多。结论:给予肝硬化大鼠穿心莲能抑制肠道细菌过度生长,减少细菌移位的发生率,对肠道黏膜屏障有保护作用,服用穿心莲可能有益于防治肝硬化肠源性细菌感染。OBJECTIVE To evaluate the effect of andrographitis on intestinal bacterial overgrowth, bacterial translocation and intestinal permeability in cirrhotic rats, and therefore to provide evidences for its clinical application. METHODS Liver-cirrhosis model in rats was induced by carbon tetrachloride. Thirty cirrhotic rats were randomly divided into four groups： group AN （n = 8, andrographitis 0. 5 g· kg^-1·d^-1 ）, group NF（n = 8, norfloxin 0. 3 g· kg^-1·d^-1）, group AN ＋ NF （n = 8, androgtaphitis 0. 5 g· kg^-1·d^-1 ＋ norfloxacin 0. 3 g· kg^-1·d^-1 ）and group CN（n = 6, 0. 9% sodium chloride 2 mL·d^-1 ）. After 2-weeks treatment, serum specimens were collected from peripheral vein and the serum levels of ALT, AST, ALB, TP, TNF-α as well as NO were determined. The mesenteric lymph nodes （MLNs）, blood, liver, spleen and contents of ileum were obtained for bacterial culture. Intestinal permeability was measured by HPLC/RID. Sections of liver and ileum were made for histological examination. RESULTS Compared with group CN, group AN, group NF and group AN ＋ NF showed decreased serum levels of NO and TNF-α （P 〈0. 05, P〈0. 01）and also significant decrease in total ileum bacterial amounts and bacterial translocation （P〈 0. 01）. Besides, the intestinal permeability in group AN and group NF was remarkably decreased （P〈0. 05）. By histological examination, obvious abnormalities were found in group CN： villi were shortened and damaged with increased inflammatory infiltration. CONCLUSION Administration of andrographitis resulted in a reduction of intestinal bacterial overgrowth, bacterial translocation and a protection of intestinal mucosal barrier, suggesting that oral administration of andrographitis may be useful in preventing cirrhotic patients from intestinal bacterium infection.

关 键 词：肝硬化 肠道通透性 肠道细菌过度生长 穿心莲 细菌移位 

分 类 号：R966[医药卫生—药理学]