乙肝病毒表面抗原基因对番茄的遗传转化及转基因番茄植株的获得  

Transformation of HBsAg Gene into Tomato and Production of Transgenic Tomato Plants

作　　者：王逸群[1] 李田[1] 

机构地区：[1]福建师范大学生命科学学院发育与神经生物学重点实验室,福州350108

出　　处：《西南大学学报（自然科学版）》2008年第7期78-83,共6页Journal of Southwest University(Natural Science Edition)

基　　金：福建省科技厅重点科技资助项目(2004N026);福建省自然基金资助项目(B0410009);福建省教育厅科技资助项目(JB03125)

摘　　要：利用农杆菌介导法将乙肝病毒表面抗原基因转入番茄,获得了转基因植株.经GUS组织化学染色、PCR扩增以及PCR-Southern检测,证明乙肝病毒表面抗原基因已转入番茄基因组中.这为番茄乙肝口服疫苗的研究奠定基础.The HBsAg gene was transformed into tomato via Agrobacterium tumefaciens and some transgenic tomato plants were obtained. GUS staining, PCR analysis and PCR-Southern analysis demonstrated that the HBsAg gene had been integrated into the tomato genome. These results might lay down a foundation for the study of oral vaccine of HBV by tomato transformed by HBsAg gene.

关 键 词：乙肝表面抗原基因 转化 转基因番茄 

分 类 号：R318[医药卫生—生物医学工程] Q943.2[医药卫生—基础医学]