反相高效液相色谱法测定小鼠血浆中德氮吡格  

Determination of TNBG in Plasma by RP-HPLC

作　　者：刘嫱[1] 陈志琼[2] 杨菲[2] 余瑜[2] 

机构地区：[1]海南医学院药学系药理教研室,571101 [2]重庆医科大学药物化学与生物材料研究室,重庆400016

出　　处：《西南大学学报（自然科学版）》2008年第7期117-120,共4页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(批准号:30371632)

摘　　要：采用固相萃取反相高效液相色谱法测定小鼠血浆中的德氮吡格。血浆样品经过C18固相萃取小柱净化后，在Lichrospher C18色谱柱（4．6mm×250mm，5μm）上，以甲醇-0．1％三乙胺水溶液（pH6．5，体积比90：10）为流动相，流速1．0mL／min，检测波长260nm对德氯吡格进行测定。实验结果表明，德氮吡格的血药浓度在1～20μg／mL范围内与峰面积呈良好线性关系（r≥0．995），平均回收率为101．36％，高中低三浓度的日内精密度均小于4％，日间精密度均小于8％，最低检测限为0．4μg／mL，该方法简便、快速、准确度高、可靠性好。A specific and simple high-performance liquid chromatographic （HPLC） method was developed for the determination of TNBG in plasma with UV-visible detection. HPLC separation on Lichrospher C18 （4.6 mm×250 mm,5μm） was carried out with an isocratic mobile phase of 90% methanol in 100 mL methanol-deionized water （pH 6.5）,containing 0.1% triethyl amine and detection at 260 nm. The retention time of TNBG was less than 6 min. The linearity was good over the concentration range 1-20 μg/mL （r≥0.995）. The recoveries were in the range 98.1-104.5% （n=5）,and the inter- and intra-day RSD were less than 8.0%. The detection limit was 0.4 μg/mL. This novel and rapid method can be employed in a routine clinical setting.

关 键 词：德氮吡格 固相萃取 反相高效液相色谱法 血浆 

分 类 号：R917[医药卫生—药物分析学]