核桃早实性相关性状的SCAR标记  

SCAR marker linked to early-bearing genes in walnut

作　　者：牛建新[1] 吕建强[1] 王林[1] 叶春秀[1] 张虎平[1] 张录霞[1] 

机构地区：[1]石河子大学农学院园艺系,新疆石河子832003

出　　处：《果树学报》2008年第5期732-735,共4页Journal of Fruit Science

基　　金：国家自然科学基金(30560090)

摘　　要：用RAPD技术对新疆核桃早实特性的分子标记进行了研究。用180个10-mer随机引物分别扩增早实和晚实近等基因池DNA筛选出5个多态性引物,结果只有引物OPG15(5′-ACTGGGACTC-3′)扩增得到1条约710bp的早实核桃特异片段。对该片段进行克隆和序列分析,并根据序列分析结果将该RAPD标记转化为重复性和特异性更好的SCAR标记。研究设计出了1对早实核桃特异SCAR引物P1(5'-ACTGGGACTCCAATTGTATC-3')和P2(5'-ACTGGGACTCTCAACTAT-3'),用这对特异引物对14份材料进行PCR扩增,结果所有晚实核桃材料无任何扩增,但早实材料均扩增出了预期大小759bp的特异带。RAPD was adopted to explore the molecular markers of early-beating genes of walnut grown in Xinjiang. 180 10mer random primers were used to amplify the DNA in early-bearing and late-bearing near isogenic pools. Five polymorphic primers were screened out of which only OPG15 （ 5＇ -ACT GGGACT-3＇） was capable of repeatedly amplifying a specific band of about 710 bp （OPG15710） from the early-bearing near isogenic pool and its individuals but not from the late bearing near isogenic pool. The specific band was cloned and sequenced,and transformed into a SCAR marker. With the specific SCAR primers P1 （5＇-ACTGGGACTCCAATTGTATC-3＇） and P2 （5＇-ACTGGGACTCTCAACTAT-3＇）, 14 materials were analyzed, and a 759 bp specific band was amplified from all the early-bearing materials but not from any of the late-bearing materials.

关 键 词：核桃 早实 晚实 RAPD SCAR 

分 类 号：S664.1[农业科学—果树学]