中国北方引种的部分越橘品种的RAPD分析  

RAPD analysis of some introduced blueberry cultivars in Northern China

作　　者：徐娜[1] 夏秀英[1] 贺建华[1] 魏立君[1] 张鲁杰[1] 

机构地区：[1]大连理工大学环境与生命学院,辽宁大连116023

出　　处：《果树学报》2008年第5期736-739,共4页Journal of Fruit Science

摘　　要：利用RAPD技术对我国北方引种的15份越橘(Vaccinium spp.)栽培品种进行了遗传多样性分析。从70条随机引物中筛选出15条引物,共扩增出94个位点,其中多态性位点83个(88.3%)。材料间遗传相似系数变化为0.5426～0.8936,表明各品种间具有一定的遗传差异。UPGMA聚类分析结果可将供试材料分为2大类、5个亚类。聚类结果与各品种的杂交系谱相对应,从分子水平揭示了品种间的亲缘关系。RAPD markers were used to assess genetic diversity in 15 blueberry （Vaccinium spp.） cuhivars. The resuhs showed that 83 （88.3%） polymorphic bands were detected among the total of 94 bands amplified by 15 screened random primers （among 70 primers）. The genetic similarity index ranged from 0.542 6 to 0.893 6. The dendrogram constructed by UPGMA showed that the 15 individuals were classified into two main groups including five subgroups. The result showed that there was a certain correlation between the clustering and the pedigrees of cuhivars.

关 键 词：越橘 栽培品种 RAPD 遗传多样性 

分 类 号：S663.6[农业科学—果树学]