鸡粪好氧发酵高效同程硝化-反硝化菌筛选及其生物学特性研究  

High-effective simultaneous nitrifying-denitrifying bacteria screening in composting of chicken manure and their characteristics

作　　者：曹立群[1] 喻其林[1] 肖维伟[1] 孙文[1] 顾文杰[1] 王立群[1] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030

出　　处：《中国农业大学学报》2008年第5期63-69,共7页Journal of China Agricultural University

基　　金：国家自然科学基金资助项目(C020305)

摘　　要：为验证鸡粪好氧发酵过程同程硝化-反硝化细菌的存在，全程进行了高效同程硝化-反硝化菌株的分离、筛选，并对筛选的高效菌株进行鉴定和生物学特性研究。筛选到1株高效同程硝化-反硝化细菌JY45—2，鉴定为假单胞菌Pseudomonas sp．，为兼性化能自养型菌株。JY45-2菌株生物学特性为：当有机物质量浓度1360mg／L、氨态氮质量浓度212mg／L、pH8、C／N为3．54和培养温度45℃时，氨态氮利用效率最高。JY45—2菌株的有机物耐受范围宽，有机物质量浓度为680～2040mg／L时，作用3d后的氨态氮利用率可达30％以上。在鸡粪好氧发酵过程中分离、筛选到1株高效同程硝化-反硝化细菌，证明了该固态发酵体系中存在同程硝化-反硝化细菌。In order to verify the existence of simultaneous nitrifying-denitrifying in composting of chicken manure, high- effective simultaneous nitrifying-denitrifying bacteria were isolated and screened. The bacteria were then identified and their biological characteristics were studied. The results showed that a high-effective simultaneous nitrifying-denitrifying strain JY45-2, belonging to facultative autotroph, was screened and identified as Pseudomonas sp. Its utilization rate to ammonium nitrogen reached the maximum in following conditions： concentration of organic compounds in 1 360 mg/ L, concentration of ammonium nitrogen in 212 mg/L, pH 8, C/N 3.54, and culture temperature at 45℃. The strain had a wide range of tolerance to organic compounds, and its utilization rate to ammonium nitrogen was above 30% when concentration of organic compounds changed from 680 mg/L to 2 040 mg/L. The study verified the existence of simultaneous nitrifying -denitrifying in this composting system.

关 键 词：鸡粪 发酵 硝化 假单胞菌 

分 类 号：S141.3[农业科学—肥料学]