苏云金芽孢杆菌杀虫晶体蛋白对棉铃虫活性分析  

Activity analysis of Cry protein from Bacillus thuringinesis against Helicoverpa armigera

作　　者：韩岚岚[1] 宋福平[2] 张杰[2] 赵奎军[1] 

机构地区：[1]东北农业大学农学院,哈尔滨150030 [2]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《东北农业大学学报》2008年第8期21-24,共4页Journal of Northeast Agricultural University

基　　金：国家“863”项目资助(2004AA214090)

摘　　要：利用已克隆的5种Bt Cry基因Cry2Ab4、Cry1Ia8、Cry1Ie1、Cry1Ca7、Cry1Cb2和1种野生菌株HD-73(Cry1Ac)表达的6种Bt Cry杀虫晶体蛋白,对棉铃虫进行生物活性分析,并将Cry1Ac杀虫晶体蛋白分别与其它5种Cry蛋白按1:1的比例组合,对棉铃虫进行生物活性测定。结果表明,单独使用Cry1Ac时对棉铃虫活性最高,LC50为3.16μg·mL-1,其次为Cry2Ab4。Cry1Ac与Cry2Ab4组合对棉铃虫也有较高的活性,LC50为48.70μg·mL-1,该组合对棉铃虫的共毒系数为1.21,有相加作用。Cry1Ac与这5种蛋白的组合对棉铃虫都有较高的毒力。The bioassay was done by using 6 Bt Cry proteins（Cry2Ab4, Cry1la8, Cry1le1, Cry1Ca7, Cry1Cb2） and protein（Cry1Ac） from a natural Bt strain（HD-73） and combination of Cry1Ac proteins and the other 5 Bt Cry proteins by 1 ： 1 against Helicoverpa armigera. The results showed that Cry1Ac had higher toxicity than other Cry proteins, the LC50 of Cry1Ac was 3.16 μg·mL^-1, followed by Cry2Ab4. The co-toxicity coefficient LC50 of Cry1A c and Cry2Ab4 combination was 48.70 μg·mL^-1 and The co-toxicity coefficient of Cry1A c and Cry2Ab4 was 1.21, which showed that the two kinds of proteins could synergize each other. Combining Cry1Ae and the other 5 Cry proteins had high toxicity to H. armigera.

关 键 词：苏云金芽孢杆菌 Cry蛋白 生物测定 棉铃虫 

分 类 号：S482.292[农业科学—农药学]