仙客来热激蛋白基因HSP21.4植物表达载体构建及鉴定  

Construction and identification of plant expression vectors containing HSP21.4 gene in Cyclamen

作　　者：车野[1] 李凤兰[1] 闻静[1] 王莹[1] 胡宝忠[1] 

机构地区：[1]东北农业大学生命科学院,哈尔滨150030

出　　处：《东北农业大学学报》2008年第8期64-69,共6页Journal of Northeast Agricultural University

摘　　要：试验以仙客来热激蛋白基因HSP21.4为材料,经PCR扩增的方法在目的片断两端添加SacⅠ位点,然后与植物表达载体pBI121连接,转化感受态大肠杆菌,经PCR和SacⅠ及XbaⅠ酶切验证,确定已将该热激蛋白基因连接到植物表达载体上,将重组质粒命名为pBI-CpHSP21.4,并采用冻融法完成了对pBI-CpHSP21.4质粒的农杆菌转化,为验证该热激蛋白基因的功能及转基因植物表达奠定基础。The experiment was conducted with HSP21.4 gene. Adding Sac Ⅰ site to the endpoints of objective genes by using PCR amplification, then ligated with pBI121. The recombinant plasmid was transferred into competent cells of Escherishia coli DH5α. The PCR and Sac Ⅰ digestion test showed that the HSP21.4 gene was constructed into plant expression vector, and recombinant plasmid designated as pBI-CpHSP21.4. The recombinant plasmid was transferred into competent cells of Agrohacteriurn tumefaciens LBA4404. One A. tumefaciens clone harboring recombinant plasmind pBI-CpHSP21.4 was obtained. It lays a foundation for testing function of HSP21.4 gene and transgenic plant expressing.

关 键 词：热激蛋白 植物表达载体 载体构建 

分 类 号：S682.262[农业科学—观赏园艺] Q813[农业科学—园艺学]