多杀菌素高产菌株的选育  

Screening of spinosads-producing strain with high yield

作　　者：车可舒[1] 王相晶[1] 向志丹[1] 

机构地区：[1]东北农业大学生命科学学院,哈尔滨150030

出　　处：《东北农业大学学报》2008年第8期74-76,共3页Journal of Northeast Agricultural University

基　　金：国家自然基金资助项目(30571234);哈尔滨市青年基金资助项目(2005AFQXJ073);东北农业大学创新团队资助项目

摘　　要：根据多杀菌素的生物合成途径对多杀菌素产生菌株刺糖多孢菌(Saccharopolyspora spinosa)进行选育,并获得了高产菌种。首先对出发菌株S-203进行了自然选育,获得效价为643μg.mL-1的菌株S-306;在紫外线诱变的基础上,筛选丙酸钠耐性变株,得到菌株S-466,摇瓶发酵单位为871 mg·L-1,比自然选育获得的出发菌株提高了35%。其传代实验表明菌株S-466的高产遗传特性稳定,为应用于发酵罐进行工业化生产奠定了基础。According to the biosynthetic pathway of spinosads produced by Saccharopolyspora spinosa, a rational selection was performed to obtain high spinosads -producing strain. Saccharopolyspora spinosa S-306 was selected with the yield of 643 μg·mL^-1 after natural selection of S-203. A strain S-466 was got after combination of UV mutation and propionate-resistance screening. Its production of spinosads reached 871 mg·L^-1, which was 35% higher than that of the ancestral strain S-466. The subculture experiments indicated that the hereditary characteristic of high productivity of S-466 was stable, which was favorable for large scale spinosads production.

关 键 词：多杀菌素 紫外诱变 丙酸盐耐性变株 

分 类 号：Q933[生物学—微生物学]