基于应用于生物传感器的新型有机-无机杂化材料  

New Organic-Inorganic Hybrid Material for Biosensor Use

作　　者：闫博[1] 赵林[1] 谭欣[1] 

机构地区：[1]天津大学化工学院,天津300072

出　　处：《传感技术学报》2008年第8期1314-1318,共5页Chinese Journal of Sensors and Actuators

基　　金：国家自然科学基金资助项目(20576092)

摘　　要：制备了一种应用于生物传感器的新型有机-无机杂化材料,这种杂化材料由二氧化硅溶胶和聚乙烯醇接枝巯基乙酸组成。此杂化材料克服了普通溶胶凝胶容易脆裂的特点,杂化材料中的巯基可以与金原子形成牢固的配位键从而使杂化材料很好地附着在金电极表面。对杂化材料进行了红外光谱、石英电子微天平、原子力显微镜分析。为了检验杂化材料的应用效果,以此杂化材料作为包埋材料在金电极上制备了葡萄糖氧化酶电极。研究了此酶电极的响应速度和稳定性,结果表明此酶电极具有较快的响应速度(响应时间为2.2s)、较好的稳定性(响应电流在70d后衰减9.6%)。A new type of sol-gel organic-inorganic hybrid material was developed and used for the production of biosensors. This material is composed of silica sol and a grafting copolymer of poly（vinyl alcohol） with thiogycolic acid. It prevents the cracking of conventional sol-gel-derived glasses, and the mercapto-group of the hybrid material can form Au-S bond with the Au atom, which can help the hybrid material adhere to the surface of Au electrode. The characters of the new grafting copolymer and hybrid material were examined by FT-IR spectrum, quartz crystal microbalance and atomic force microscope. To demonstrate the new hybrid material＇s application, glucose oxidase was immobilized in this hybrid material to fabricate a glucose oxidase biosensor on Au electrode. The biosensor exhibited remarkable short response time （ 2. 2 s ） and long-term stability in discontinuous-operation mode （9. 6 % decline of response current after 70 days）.

关 键 词：聚乙烯醇接枝巯基乙酸 有机-无机杂化材料 生物传感器 葡萄糖氧化酶电极 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置]