超临界CO_2萃取灵芝子实体中的三萜类成分  

Extraction of triterpenoids from fruits of Ganoderma lucidum by supercritical fluid extraction

作　　者：宋师花[1] 贾晓斌[1] 陈彦[1] 陈斌[1] 王丽静[2] 赵呈雷[1] 

机构地区：[1]江苏省中医药研究院中药新型给药系统应用技术重点研究室,江苏南京210028 [2]江苏大学,江苏镇江212013

出　　处：《中国中药杂志》2008年第17期2104-2107,共4页China Journal of Chinese Materia Medica

基　　金：江苏省公益专项(BM2007701)

摘　　要：目的:筛选超临界CO2萃取灵芝子实体中三萜类成分的最佳工艺条件。方法:采用RP-HPLC测定灵芝酸B的含量、可见分光光度法测定灵芝总三萜含量,设计正交试验优化超临界CO2萃取灵芝子实体中三萜类成分的工艺条件。结果:超临界CO2萃取灵芝子实体中三萜类成分的最佳工艺条件:萃取压力为25 MPa,萃取温度为45℃,萃取时间为1.5 h,夹带剂(95%乙醇)用量为3 mL.g-1。结论:超临界CO2萃取灵芝子实体中三萜类成分具有萃取效率高、萃取时间短、操作简单等优点,其方法可靠,切实可行。Objective： To optimize the extracting condition for triterpenoids from the fruits of Ganoderma lucidum by supercritical fluid extraction （SFE）. Method： Optimum extraction conditions were studied by orthogonal tests. The total triterpenoids were determined by ultraviolet spectrophotometry and ganoderic acid B was determined by RP-HPLC. Result： The optimal extraction conditions were that the pressure 25 MPa, the temperature was 45 ℃, the extraction time was 1.5 hour, and the ethanol was employed as modifier carrier at the volume of 3 mL · g^-1. Conclusion： SFE is superior and it is feasible to extract triterpenoids from fruits of Ganoderma lucidum.

关 键 词：灵芝 超临界CO2萃取 正交试验 灵芝总三萜 灵芝酸B RP—HPLC 

分 类 号：R284.1[医药卫生—中药学]