紫金莲药材的质量控制方法的研究  

Study on qualitative and quantitatire methods for Caratostigma willmottianum

作　　者：王爱民[1] 王永林[1] 李勇军[1] 苏红[1] 赵平[1] 

机构地区：[1]贵阳医学院药学院,贵州贵阳550004

出　　处：《中国中药杂志》2008年第17期2130-2132,共3页China Journal of Chinese Materia Medica

基　　金：国家"十一五"科技支撑计划项目子课题(2006BAI06A01-3);贵州省中药现代化科技产业研究开发专项基金项目(黔科合中药专字[2006]5042号)

摘　　要：目的：建立紫金莲药材的定性鉴别和定量检测方法。方法：采用TLC法，以紫金莲对照药材和白花丹醌对照品鉴别紫金莲药材；以HPLC测定白花丹醌含量。结果：采用C18色谱柱，以甲醇-水（75：25）为流动相，检测波长265nm，供试品色谱中白花丹醌分离良好，白花丹醌进样量在33．6～313．6ng线性关系良好，r=0．9999，平均加样回收率100．3％，RSD1．9％。结论：通过研究制定了紫金莲药材TLC和HPLC质量控制方法。Objective： To establish the qualitative and quantitative methods of Caratostigma willmottianum. Method： The roots of Radix Ceratostigmae were identified by TLC with the authentic medicinal material and plumbagin as the reference. The contents of plumbagin in Radix Ceratostigmae were determined by HPLC. Result： The TLC method was simple and specific. A Diamonsil C18 column was used. The mobile phase was methanol-water （75： 25）. Plumbagin in the sawple extract was separated well. The linear range of plumbagin was 33.6 - 313.6 ng. The average recovery of plumbagin was 100. 3% and RSD was 1.9%. Conclusion： The methods can be used for identification and determination of plumbagin in C. willmottianum.

关 键 词：紫金莲 薄层色谱 白花丹醌 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]