姜黄素对大鼠油酸型急性肺损伤的保护作用  

Protective effect of curcumine on oleic-induced acute lung injury in rats

作　　者：朱瑞芳[1] 周敏[1] 何建林[1] 丁福云[1] 余书勤[1] 绪广林[1] 

机构地区：[1]南京师范大学生命科学学院江苏省医药超分子材料及应用重点实验室江苏省分子医学生物技术重点实验室,江苏南京210046

出　　处：《中国中药杂志》2008年第17期2141-2145,共5页China Journal of Chinese Materia Medica

基　　金：江苏省高校自然科学基金重点项目(06KJA31024)

摘　　要：目的:研究姜黄素对大鼠油酸型急性肺损伤(ALI)的保护作用,探讨其作用机制。方法:动物随机分为空白组、模型组、姜黄素组(剂量分别为5,10,20 mg.kg-1)、地塞米松组1 mg.kg-1。制备大鼠油酸型ALI模型,以动物肺功能测定仪测定动态肺顺应性(Cdyn)的变化,利用光镜观察肺部组织形态学变化,称重法计算肺指数及湿/干比(W/D),紫外分光光度法检测肺组织微血管的渗透性和支气管肺泡灌洗液(BALF)中蛋白含量,酶联免疫吸附法(ELISA)检测BALF中肿瘤坏死因子-α(TNF-α),白介素6(IL-6)和白介素10(IL-10)的含量。结果:姜黄素可明显抑制Cdyn降低,改善大鼠肺功能,降低肺指数及湿/干重比,降低肺渗透性,降低BALF中蛋白及TNF-α(250.4±21.6 vs 172.53±14.88,122.2±10.98,108.69±3.39)ng.L-1,IL-6(763.6±88.33 vs 207.41±15.55,172.13±21.91,142.92±4.32)ng.L-1的水平,提高IL-10(98.90±2.99 vs 208.44±16.30,218.43±6.23,252.70±20.58)ng.L-1的含量,减轻肺组织病理学损伤。结论:姜黄素对油酸型ALI具有保护作用,其可能机制为调节致炎因子与抗炎因子。To investigate the effect of curcumine on acute lung injury induced by oleic acid in rat and the possible mechanism of action. The rats were divided into 6 groups randomly： normal group, control group, curcumine groups （5, 10, 20 mg · kg^-1） and dexamethasone group （ 1 mg · kg^- 1 ）. During the experiment, acute lung injury was induced by oleic acid in rat. The changes of dynamic lung compliance were recorded by anrise 2005 pulmonary function test apparatus, light microscope was used to examine histological changes and lung index as well as wet to dry weight ratio was calculated by weighting method. Lung vascular permeability and protein level in BALF were detected by ultraviolet spectrophotometry, and the concentrations of TNF-c~, IL-6 and IL-10 in BALF were measured by enzyme linked immunosorbent assay （ELISA）. The result showed that the changes of pulmonary compliance were inhibited and pulmonary function was improved by curcumine. The OA-induced elevation of lung index was restrained, as well as wet to dry weight ratio, lung vascular permeability, protein level, TNF-α （250. 4 ±21.6 vs 172.53 ±14. 88, 122. 2 ± 10. 98, 108.69±3.39） ng · L^-1, IL-6 （763.6 ± 88. 33 vs 207. 41 ± 15.55, 172. 13 ± 21.91, 142.92 ± 4. 32） ng ·L^-1 in BALF in curcumine groups, IL-10 （98.90 ±2. 99 vs 208.44 ± 16. 30, 218.43 ± 6. 23, 252. 70± 20. 58） ng · L^-1 in BALF was increased, respectively significantly. Light microscope findings shown that the impairment in curcumine groups was far less severe than that in model groups. Pretreatment of curcumine showed beneficial effect on acute lung injury induced by oleic acid in rats. The mediation of both prointlammatory factor and antlnflammatory factor by curcumine may be involved in mechanism of action of curcumine effects.

关 键 词：姜黄素 急性肺损伤 肺功能 支气管肺泡灌洗液 

分 类 号：R285.5[医药卫生—中药学]