荆芥挥发油对大鼠胸腔白细胞5-脂氧酶活性的影响  

Effect of herba schizonepetae volatile oil (STO) on activity of 5-lipoxygenase in rat thoracic cavity leukocytes

作　　者：赵璐[1] 曾南[1] 唐永鑫[1] 刘晓帅[1] 杨旋[1] 瞿礼萍[1] 

机构地区：[1]成都中医药大学药理教研室,四川成都611137

出　　处：《中国中药杂志》2008年第17期2154-2157,共4页China Journal of Chinese Materia Medica

基　　金：四川省2004年度杰出青年基金(2004QN13);2005年度人事部留学归国人员课题

摘　　要：目的：探讨荆芥挥发油对5-脂氧酶（5-LO）活性的影响，以期进一步阐明其干预花生四烯酸代谢的抗炎机制。方法：以角叉菜胶急性胸膜炎模型大鼠的胸腔白细胞为基本反应体系，分别加入外源性花生四烯酸（AA）与不同质量浓度的荆芥挥发油（0．011，0．022，0．043，0．087，0．179，0．255，0．364g·L^-1）、齐留通（0．625×10^-3g·L^-1），在钙离子载体A23187诱导下，提取完整白细胞的5-LO代谢产物白三烯B4（LTB4）和5-羟基二十碳四烯酸（5-HETE），运用反相高效液相色谱（RP—HPLC）紫外检测分离测定LTB4和5-HETE水平。结果：荆芥挥发油在0．022—0．364g·L^-1内，对大鼠胸腔白细胞LTB4与5-HETE的生物合成均呈现显著性的抑制作用（P〈0．05或P〈0．001），并表现出剂量依赖性，其对LTB4与5-HETE生成的IC50分别为0．124g·L^-1和0．142g·L^-1。结论：荆芥挥发油体外可剂量依赖性地抑制大鼠胸腔白细胞花生四烯酸代谢酶5-LO的活性，表明其良好的抗炎作用与抑制5-LO活性，减少致炎物质LTB4的生成有关。Objective： To investigate the effect of herba schizonepetae volatile oil （STO） on the activity of 5-lipoxygenase （5- LO）, so as to elucidate its mechanisms of anti-inflammatory action which is related to the arachidonic acid （AA） metabolism. Method： Thoracic cavity leukocytes from the pleurisy model rat induced by injecting 1%-carrageenan into the pleural cavity were collected. Then 0. 4 mL cell suspension including 2 × 10^7 cells per millilitre were used as the reaction system in vitro. STO in different concentrations （final concentration 0. 011, 0. 022, 0. 043, 0. 087, 0. 179, 0. 255, 0. 364 g · L^-1 ）, zileuton（final concentration 0. 625 × 10^-3 g · L^-1 ）, and DMSO in the same volume were added into the reaction tube respectively. The reaction tubes were incubated at 37 ℃ for 20 rain and CaCl2 （final concentration 2 mmol · L^-1 ）, MgCl2 （final concentration 0. 5 mmol · L^-1 ）, exogenous AA （final concentration 200 μmol · L^-1） and A23187 （final concentration 5 μmol · L^-1） were added in turns during this period. The reaction tubes were mixed and continu- ously incubated at 37 ℃ for 30 min. After terminating reaction by adding methanol, the metabolites of 5-LO, leukotriene B4 （LTB4 ） and 5-hydroxy-6, 8, 11, 14-eicosatetraenoic acid （5-HETE）, were extracted, separated and detected by means of RP-HPLC. Result： Compared with control group, STO significantly inhibited the biosynthesis of LTB4 and 5-HETE at final concentration between 0. 022 g · L^-1 and 0. 364 g · L^ -1 （P 〈0. 05 or 0. 001 ） in dose dependence manner, and its IC50 value was 0. 124 g · L^ -1 and 0. 142 g · L^ -1 for LTB4 and 5-HETE, respectively. Conclusion： STO can inhibited the activity of 5-LO, which is an important enzyme of AA metabolism, in rat thoracic cavity leukocytes in a dose-dependent manner in vitro. It is suggested that the mechanism of anti-inflammatory action of STO is related to its inhibiting the activity of 5-LO and decreasing the level of major inflammat

关 键 词：荆芥挥发油 5-脂氧酶 白三烯B4 5-羟基二十碳四烯酸 反相高效液相色谱 

分 类 号：R285.5[医药卫生—中药学]