抗穗萌药剂组合的筛选及其抑制种子发芽的同功酶电泳研究  

The Selection of Inhibitors Combination to Inhibit Pre-harvest Sprouting In Rice and Study on Eletrophoretic Analysis

作　　者：王业文[1] 张莹[1] 靳西彪[1] 魏毅[1] 朱建清[1] 

机构地区：[1]四川农业大学水稻研究所,四川温江611130

出　　处：《种子》2008年第8期18-21,共4页Seed

基　　金：国家科技支撑项目(200703402800);四川省国际科技合作项目(2006H12-016)

摘　　要：采用三因素五水平二次回归正交旋转组合设计,研究青鲜素、多效唑、烯效唑组合对种子萌发的影响并进行该药剂组合抑制种子发芽的同功酶电泳分析。结果表明,对种子萌发影响由大到小依次是青鲜素、烯效唑、多效唑,最佳的药剂组合是青鲜素、多效唑、烯效唑的浓度分别为9.55、95.46、1104.54mg/L。理论上经该药剂组合处理后,未萌发的种子为62%,远高于对照。在成熟期、黄熟期喷施本抑制剂后,G46A、D62A种子的淀粉酶同功酶酶带恢复到正常生长的同功酶水平,该药剂基本上消除了由喷施"920"带来的淀粉酶大量合成效应。而该药剂对正常发芽种子的淀粉酶同功酶酶带没有抑制效应。因此推测该药剂抑制种子发芽的机制可能是通过抑制淀粉酶同功酶酶带的合成从而达到抑制或延缓种子发芽。Quadratic orthogonal regressive rotation design of three factors was applied to study the effect of MH,PP333,S-07 on seed germination and Eletrophoretic Analysis of inhibitors to inhibit amylase. The result showed that the effect of inhibitor on seed germination is MH 〉 S-07 〉 PP333 The best combination is as following MH 9.55 mg/L ＋ PP333 95. 46 mg/L ＋ S-07 1 104.54 mg/L. In theory, the percent of ungerminated seed is 62% by the inhibitor combination treatment, that is highly more than ck. The band of Amylase in seed of G 46 A and D 62 A is same as that of ck by the inhibitor combination treatment in mature stage ; whereas the inhibitor combination is effective to inhibit seed spayed GA germinated. The band of Amylase in geminated seed is not inhibited by the inhibitor combination, so the mechanism of the inhibitor combination inhibiting the seed germination may be inhibit the synthesis of the band of Amylase to inhibit or delay the seed germination.

关 键 词：水稻 正交回归旋转设计 抗穗萌药剂 同功酶 

分 类 号：S965.1[农业科学—水产养殖] S641.3[农业科学—水产科学]