三种卟啉类光敏剂的荧光量子产率  

Determination of fluorescence quantum yields of porphyrin-based photosensitizers

作　　者：余松林[1] 林黎升[1] 林慧韫[1] 李步洪[1] 谢树森[1] 

机构地区：[1]福建师范大学医学光电科学与技术教育部重点实验室,福建省光子技术重点实验室,福建福州350007

出　　处：《光电子．激光》2008年第9期1283-1286,共4页Journal of Optoelectronics·Laser

基　　金：福建省自然科学基金重点资助项目(2008J0001);福建省闽港人才合作重点资助项目

摘　　要：实验研究了喜泊分(Hiporfin),血啉甲醚(HMME)和癌光啉(PsD-007)等3种国产卟啉类光敏剂在水溶液中的吸收和荧光光谱特性,并利用对照法首次测定了它们在水溶液中的荧光量子产率。Hiporfin,HMME和PsD-007具有相似的吸收光谱和荧光发射特性,它们的荧光量子产率分别为0.013,0.026和0.020。实验表明光敏剂的荧光量子产率还与溶液中的氧含量有关。实验结果为光动力学诊断中光敏剂的选择,以及相应最佳荧光激发和检测波长的选取提供了理论依据。The absorption and fluorescence spectral characteristics of hematoporphyrin derivative （Hiporfin）, hematoporphyrin monomethyl ether （HMME） and photocarcinorin （PsD-007） were comparatively investigated, and their fluorescence quantum yields were further evaluated by the relative comparison measurement for the first time. A linear relationship between the integrated fluorescence intensity and the absorbance of porphyrin-based photosensitizers was observed. The fluorescence quantum yields of Hiporfin, HMME and PsD-007 were detemined to be about 0. 013,0. 026 and 0. 020, respectively. These results are crucial for choosing the porphyrin-based photosensitizer for photodynamic diagnosis,and then detemining for the optimal excitation and fluorescence detection wavelengths for the photosensitizer used.

关 键 词：卟啉类光敏剂 光谱特性 荧光量子产率 光动力学诊断 

分 类 号：O657.3[理学—分析化学]