棉花6个小分子质量热激蛋白基因的序列、表达与定位  

Cloning,Expression,and Mapping of Six Low-Molecular-Weight HeatShock Protein Genes in Cotton

作　　者：贺亚军[1] 郭旺珍[1] 张天真[1] 

机构地区：[1]南京农业大学棉花研究所/作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《作物学报》2008年第9期1574-1580,共7页Acta Agronomica Sinica

基　　金：国家重点基础研究发展计划(973计划)项目(2002CB111301);教育部长江学者和创新团队发展计划项目(IRT0432);高等学校创新引智计划项目(B08025)

摘　　要：利用棉花纤维cDNA文库,分离到6个小分子质量的热激蛋白基因。序列结构分析发现,它们分别属于3种不同类型的小热激蛋白。RT-PCR分析表明,它们在棉花体内具有不同的转录表达特征,可能行使不同的功能,它们的转录与棉花特定的发育阶段相关,线粒体小热激蛋白和细胞质Ⅰ类小热激蛋白基因受纤维启始和分化的调控,而细胞质Ⅱ类小热激蛋白与棉花叶片的生长发育相关。利用本实验室的四倍体棉花遗传图谱,对这6个小热激蛋白基因进行定位,其中3个被定位在A4、D8和A6染色体上。Heat-shock proteins （HSPs）, known as stress proteins, are created when cells are exposed to elevated temperatures, or to other kinds of environmental stress. Although HSPs were first characterized because their expression increased in response to elevated temperature, some of them are found in normal, nonstressed cells. HSPs have many biochemical functions in cell metabolism during plant developmental process. The objective of the study was to isolate low-molecular-weight HSP genes, further put a foundation to illustrate them function in cotton fiber developmental stages. Six low-molecular-weight HSP genes wcre isolated from cotton fiber cDNA library. Sequence analysis showed that they belong to three different types of low-molecular-weight HSP. RT-PCR analysis revealed that they exhibit different transcripional profile in cotton. The mitochondrial and cytoplasmic I HSP genes were regulateded by the fiber initiation; however, cytoplasmic II HSP genes were more closely correlated with the growth and development of cotton leaf. Using genetic map of the tetraploid cotton constructed by our laboratory, three of these genes, LMWHSP1, LMWHSP2, and LMWHSP3, were mapped on the cotton chromosomes A4, D8, and A6, respectively.

关 键 词：棉花 小分子质量热激蛋白 表达 基因定位 

分 类 号：S562[农业科学—作物学] O633.1[理学—高分子化学]