蒿甲醚体外抗弓形虫作用的实验研究  

EXPERIMENTAL STUDY ON THE EFFECT OF ARTEMETHER AGAINST TOXOPLASMA

作　　者：刘杨[1] 张永浩[1] 李哲[1] 姚向民[2] 

机构地区：[1]西安医科大学寄生虫学教研室,西安710061 [2]西安医科大学组织胚胎学教研室,西安710061

出　　处：《中国寄生虫学与寄生虫病杂志》1997年第6期366-369,共4页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：目的 :了解蒿甲醚体外抗弓形虫速殖子的作用效果。方法 :采用大鼠肾细胞培养技术 ,观察蒿甲醚 (浓度 0 .0 1- 10 0 μg/ ml)对虫体侵入细胞及在细胞内增殖的影响 ,选乙酰螺旋霉素作对照。结果 :蒿甲醚抗弓形虫作用的最低有效浓度为 0 .1μg/ ml。当其浓度为 4 μg/ ml时 ,作用速殖子 4 8h后 ,光镜下多数虫体变形 ,胞质内出现空泡、颗粒。乙酰螺旋霉素的作用不及相同浓度的蒿甲醚。结论 :蒿甲醚在体外能抑制弓形虫速殖子的侵袭和繁殖力 ,且具有杀虫效果。AIM:To study the activities of artemether(Art) against the tachyzoite of Toxoplasma gondii in vitro.METHODS:The method of cell culture of rat kidney was used for investiga- tion the effects of Art(concentration0 .0 1 - 1 0 0 μg/ml) on the invasion and proliferation of T.gondii.Acetylspiramycin was used as control.RESULTS:The minimum effective concen- tration of Artagainst T.gondii was0 .1 μg/ml.When the concentration of Artwas4μg/ml, most of the Toxoplasma organisms appeared deformed and distorted after48h with vacuoles and granules in its cytoplasm under light microscope.The anti Toxoplasma effect of acetyl- spiramycin was inferior to that of Art at the same concentration.CONCL USION:Art could inhibit the invasion and multiplication of T.gondii tachyzoites and kill them in vitro.The toxoplasmacidal effect of Art was proved to be superior to that of acetylspiramycin.

关 键 词：蒿甲醚 弓形体 弓形体病 

分 类 号：R531.805[医药卫生—内科学] R978.6[医药卫生—临床医学]