细粒棘球蚴生发细胞体外培养的实验观察  

IN VITRO CULTIVATION OF THE GERMINAL CELLSFROM LARVAL ECHINOCOCCUS GRANULOSUS

作　　者：陆家海[1] 程维兴[1] 郭中敏[2] 冯德元[1] 陈启军[3] 李德昌[3] 郭固[2] 

机构地区：[1]兰州军区乌鲁木齐总医院,乌鲁木齐830000 [2]新疆畜牧科学院,乌鲁木齐830000 [3]解放军农牧大学,长春130062

出　　处：《中国寄生虫学与寄生虫病杂志》1997年第6期392-394,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：军队‘九五’青年基金!资助项目 (No.96 Q0 2 3)

摘　　要：目的 :为培育人源细粒棘球蚴细胞系 (株 ) ,以供用于免疫预防研究。方法 :用外科方法从临床确诊的细粒棘球蚴患者的肝脏中取出包囊 ,以生发层和原头节为培养材料 ,采用RPMI1640、199和改良 DMEM培养液 ,用鼠尾胶原蛋白包被和不包被的培养瓶 ,对其进行初培养 ,并进行对比观察。结果 :以胶原蛋白作为支撑材料 ,应用改良 DMEM培养液培养的人源细粒棘球蚴生发层和原头节效果优于其他 ,已成功地培育了人源细粒棘球蚴细胞系 ,并传至第 2 0代。原代培养时间需 2 8d- 4 5d,3代以内细胞为多形态性。结论 :建立了适合人源细粒棘球蚴细胞体外培养的方法。AIM:To establish a cell line of larval Echinococcusgranulosus.METHODS:The germi- nal cells and protoscoleces were obtained surgically from the patient with hydatidosis.The materials of germinal layer and protoscoleces were perpared for primary cultures.Trypsin was used for the enzymatic release of the monodispersed cells from the protoscoleces.The culture media were RPMI1 6 40 ,1 99and improved DMEM with1 5 % - 2 0 % fetal calf serum. RESULTS:Using improved DMEM medium and rat- tail collagen- coated flasks were suitable for cells on growh of larval Echinococcusgranulosus,and was established cell line successful- ly.It has been 2 0 passages up to now.The times of primary cultures needed 2 8- 4 5 day. The cultured cells atpassage1 - 3were varying in size and shape.CONCLUSION:A method was established for culture cells of larval Echinococcus granulosus from patient.

关 键 词：人源 细粒棘球蚴 细胞培养 细胞系 

分 类 号：R383.33[医药卫生—医学寄生虫学]