吗啡哌替啶芬太尼对离体人红细胞膜ATPases活性的影响  

A Comparative Study of the Effects of Morphine Pethidine and Fentanyl on Human Erythrocyte Membrane ATPases Activities in Vitro

作　　者：尹宁[1,2] 钱燕宁[1,2] 林桂芳 张国楼 

机构地区：[1]江苏省镇江市第一人民医院 [2]南京医科大学第一附属医院

出　　处：《临床麻醉学杂志》1997年第6期343-344,共2页Journal of Clinical Anesthesiology

基　　金：国家自然科学基金

摘　　要：目的：比较研究吗啡、哌替啶、芬太尼对离体人红细胞膜ＡＴＰａｓｅｓ活性的影响。方法：将不同浓度的药物分别与人红细胞孵育，以孔雀绿－磷钼酸复合比色法测定ＡＴＰａｓｅｓ活性。结果：随着药物浓度的增加，吗啡对Ｎａ＋，Ｋ＋－ＡＴＰａｓｅ活性有增强作用（Ｐ＜０．０５），芬太尼则呈明显地抑制作用（Ｐ＜０．０５），哌替啶仅具有轻微抑制作用；吗啡能明显抑制Ｍｇ２＋－ＡＴＰａｓｅ和Ｃａ２＋，Ｍｇ２＋－ＡＴＰａｓｅ活性（Ｐ＜０．０１或Ｐ＜０．０５），但哌替啶和芬太尼对Ｍｇ２＋－ＡＴＰａｓｅ活性均无影响；这两种药物仅轻微地抑制Ｃａ２＋，Ｍｇ２＋－ＡＴＰａｓｅ活性。结论：应用大剂量吗啡或芬太尼时，需注意他们对人红细胞膜ＡＴＰａｓｅｓ活性的影响。Objective: A comparative study of the effects of morphine, pethidine and fentanyl on human erythrocyte membrane ATPases activities was performed in vitro. Methods: The erythrocytes were incubated in different concentrations of the drugs studied respectively. The ATPases activities were measured by malachite greenpnosphatomolybdic acid colorimetric method. Results: The Na+, K+ATPase activity was significantly increased as the concentration of morphin was increased (P<005), but inhibited as that of fentanyl(P<005) and slightly inhibited by pethidine(P>005). Mg2+ATPase and Ca2+, Mg2+ATPase activities were significantly inhibited by morphine(P<001, or P<005), but Mg2+ATPase activity was not influenced by either pethidine or fentanyl. Ca2+, Mg2+ATPase activity was inhibited slightly by either pethidine or fentanyl(Both P>005). Conclusion: Attention should be paid to the influence of morphine and fentanyl on the human erythrocyte membrane ATPases activity when they are used in large dose.

关 键 词：吗啡 哌替啶 芬太尼 ATP酶 

分 类 号：R971.2[医药卫生—药品]