HBs/CD3双特异性抗体介导LAK对HBV DNA转染细胞的细胞毒作用  

THE CYTOTOXICITY OF HBs/CD3 BISPECIFIC MONOCLONAL ANTIBODY TARGETED LAK CELLS ON HEPG2 CELLS TRANSFECTED BY HEPATITIS B VIRUS DNA

作　　者：楼敏[1] 皇甫玉珊[1] 程云[1] 刘洪[1] 谢建芳[1] 

机构地区：[1]解放军第302医院

出　　处：《解放军医学杂志》1997年第6期397-399,共3页Medical Journal of Chinese People's Liberation Army

摘　　要：应用化学偶联方法合成ＨＢｓ／ＣＤ３双特异性抗体，并观察其对ＬＡＫ细胞的细胞毒性作用的影响。用化学偶联剂ＳＰＤＰ将鼠源的ＣＤ３与ＨＢｓ单克隆抗体连接得到ＨＢｓ／ＣＤ３双特异性抗体；设ＨｅｐＧ－２和Ｋ５６２细胞对照组。实验显示，ＨＢｓ／ＣＤ３双特异性单克隆抗体能显著提高ＬＡＫ细胞与２．２．１５细胞结合率；应用１２５ＩＵｄＲ释放试验检测细胞毒性，发现双特异性抗体能增强ＬＡＫ细胞对２．２．１５细胞的细胞毒性，并与抗体浓度相关，ＢｓＡｂ浓度递增，细胞毒性作用随之增加。对细胞毒性作用的特异性研究表明：加入ＨＢｓ／ＣＤ３ＢｓＡｂ后，ＬＡＫ细胞对２．２．１５细胞毒性显著高于ＨｅｐＧ－２和Ｋ５６２细胞组。结果表明，ＨＢｓ／ＣＤ３双特异性抗体具有双向识别ＬＡＫ细胞和２．２．１５细胞的特性，特异地促进ＬＡＫ细胞与靶细胞结合，发挥选择性细胞毒性作用。Bispecific monoclonal antibodies were recently applied in targeting lymphokine activated killer (LAK) cells against hepatoma cells, gastroma cells, HIV infected cells and so on. We observed the regulatory effects of HBs/CD3 bispecific monoclonal antibody targeted LAK cells on HepG2 cells transfected by hepatitis B virus HBV DNA (2.2.15 cells ). CD3 McAb and HBs McAb were crosslinked with Nsuccinimidyl 3[2pyridldithil] proprionate. The bispecific character was identified with undirected immunofluorescent staining. The cytotoxic activity of LAK cells on 2.2.15 cells was determined by 125IUdR release assay was compared with HepG2 cells and K562 cells. The results showed that HBs/CD3 bispecific antibodies could bind to 2.2.15 cells as well as LAK cells and could significantly enhance the lysis of HerG2 cells transfected by hepatitis B virus DNA. It was concluded that HBsAb could specially combine LAK cells with 2.2.15 cells and selectively increase cytotoxicity of LAK cells.

关 键 词：杀伤细胞 淋巴因子激活 乙型肝炎病毒 转染 

分 类 号：R392.11[医药卫生—免疫学] R373.21[医药卫生—基础医学]