Nogo-66受体单链RNA干扰对大鼠脊髓半切损伤的治疗效应  

Therapeutic effect of NgR siRNA on nerve regeneration following spinal cord hemi-transsection injury in rats

作　　者：包映晖[1] 沈剑虹[2] 梁玉敏[1] 罗其中[1] 江基尧[1] 

机构地区：[1]上海交通大学医学院附属仁济医院神经外科,200127 [2]南通大学附属医院神经外科

出　　处：《中华创伤杂志》2008年第9期696-699,共4页Chinese Journal of Trauma

基　　金：国家自然科学基金资助项目（30371456）;上海市科委科技攻关资助项目（034119822）

摘　　要：目的观察Nogo-66受体（NgR）单链RNA干扰（siRNA）对大鼠脊髓半切损伤的治疗效应。方法建立大鼠脊髓半切损伤模型，脑室内注射筛选出的有效NgR siRNA，采用运动功能评分、辣根过氧化物酶（HRP）逆行示踪、HE染色等方法，评价NgR siRNA对损伤脊髓的治疗效应。结果神经功能评分示，从第4周起，siRNA治疗组的运功功能恢复好于等渗盐水组和空白对照组，但差异无统计学意义。HRP逆行神经示踪示，治疗组多数可于脊髓前角见较多的标记细胞，与等渗盐水组和空白对照组比较，差异有统计学意义（P〈0．05）。HE染色示脊髓损伤后8周，等渗盐水组和对照组的损伤区纤维排列紊乱，siRNA组部分大鼠损伤区见有较连续的纤维通过。结论NgR siRNA可促进脊髓损伤后的神经再生修复。Objective To investigate the therapeutic effect of NgR siRNA on nerve regeneration following spinal cord hemi-transsection injury in rats. Methods Rats with Ts spinal cord hemi-transsection were divided into 3 groups, ie, siRNA group, NS group and control group. SiRNA or NS was injected into lateral cerebral ventricle just after spinal cord injury. The therapeutic effect of NgR siRNA was evaluated by using BBB locomotor rating scale, retrograde horseradish peroxidase （HRP） tracing and HE staining. Results BBB locomotor rating scale showed that the recovery of the locomotor function of siRNA group seemed to be better than that of the other two groups from the 4th week, but there was no statistical difference. Retrograde HRP tracing showed a large number of positive cells in the anterior horn of spinal cord, with statistical difference compared with NS group and control group （ P 〈 0. 05 ）. Eight weeks after spinal injury, HE staining showed disorderly distribution of the fibres in NS group and control group but serial fibres in the injury region in siRNA group. Conclusion NgR siRNA may promote the nerve regeneration following spinal cord injury.

关 键 词：脊髓损伤 受体 Nogo蛋白 RNA干扰技术 

分 类 号：R651.2[医药卫生—外科学]