高氧液对颅脑交通伤家兔神经细胞凋亡的影响  

Neuron apoptosis in response to hyperoxic liquid in rabbit with traffic brain injury

作　　者：陈大庆[1] 朱烈烈[1] 

机构地区：[1]温州医学院附属第二医院急诊科,325027

出　　处：《中华创伤杂志》2008年第9期740-742,共3页Chinese Journal of Trauma

摘　　要：目的探讨静脉输注高氧液对颅脑交通伤家兔神经细胞凋亡的影响。方法筛选合适的撞击速度制作家兔颅脑交通伤模型成功后，每日分别给予高氧液25，50，75，100ml治疗。观察各组动物的线粒体呼吸控制率（RCR）、膜电位（MMP）、神经细胞凋亡率的改变，以及神经损害程度评分（NSS）的变化。结果经过筛选，以72km／h的撞击速度制作的脑损伤模型为研究对象，与未干预组相比，对照组和治疗组脑线粒体MMP、RCR、磷氧比（P／O）及氧化磷酸化率（OPR），神经细胞凋亡率和NSS差异均有统计学意义（P〈0．05）。100ml高氧液治疗组的MMP、RCR、P／O及OPR水平要优于75ml高氧液治疗组（P〈0．05），而神经细胞凋亡率和NSS则低于75ml高氧液治疗组，但差异无统计学意义（P〉0．05）。结论静脉输注高氧液能降低颅脑损伤后神经细胞凋亡率，通过干预线粒体功能可能是高氧液影响神经细胞凋亡从而改善预后的重要途径之一。Objective To investigate the effect of hyperoxic liquid on neuron apoptosis in rabbit with traffic brain injury. Methods Rabbit models of brain injury were established by screening suitable striking intensity and supplied respectively with hyperoxic liquid for 25, 50, 75 and 100 ml each day to observe the respiratory control rate （RCR） of mitochondria, mitochondria membrane potential （MMP）, apoptosis rate of neurocytes and NSS grade. Meanwhile, non-intervention group and control groups were set. Results The rabbit model made by striking velocity of 72 km/h was selected as the study object. MMP, RCR, phosphate/oxygen ratio （P/O） , oxygenated phosphate rate （OPR） and apoptosis rate of neurocytes and NSS grade in control and treatment group showed statistical difference compared with non-intervention group （P 〈 0.05 ）. MMP, RCR, P/O and OPR in 100 ml hyperoxic liquid treat- ment group were higher than those in 75 ml hyperoxic liquid treatment group （ P 〈 0.05 ）, but the apoptosis rate of neurocytes and NSS grade showed no statistical difference （P 〉 0.05）. Conclusion Intravenous hyperoxic liquid infusion can decrease apoptosis rate of neurocytes, as indicates that hyperoxic liquid affects apoptosis of neurocytes and hence ameliorates prognosis mainly by intervening respiratory function of mitochondria.

关 键 词：脑损伤 事故 交通 线粒体 凋亡 高氧液 

分 类 号：R651.1[医药卫生—外科学]