大鼠神经干细胞生长及增殖规律的体外研究  

The study on growth and proliferation of neural stem cells from rats in vitro

作　　者：陈请国[1] 付勇[2] 王显红[3] 吴小慧[4] 朱锐[5] 李泽文[1] 龚树生[6] 

机构地区：[1]华中科技大学同济医学院附属协和医院耳鼻咽喉科,武汉430022 [2]浙江大学附属第一医院耳鼻咽喉科 [3]咸宁学院附属第一医院耳鼻咽喉科 [4]华中科技大学同济医学院附属协和医院检验科 [5]华中科技大学同济医学院附属协和医院中西医结合科 [6]首都医科大学附属北京同仁医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉头颈外科杂志》2008年第16期747-750,共4页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：国家自然科学基金资助项目(No:30672308)

摘　　要：目的：体外无血清条件下培养胚鼠神经干细胞，观察其生长及分化情况。方法：取孕16～18d SD系大鼠的胚胎海马组织，在含EGF、bFGF和B27的DMEM／F12培养基中培养，电子显微镜下观察其增殖分化过程，并通过免疫荧光方法鉴定分化后的细胞类型。结果：神经干细胞在无血清培养基中生长旺盛，8d左右就可以形成胞体透亮、折光性好的干细胞球，分化后的细胞显示NSE、GFAP免疫阳性。结论：无血清培养条件下神经干细胞生长良好，而在含血清的培养基中可以分化为神经元和星形胶质细胞。Objective：To investigate culturing neural stem cells（NSCs） from rat embryos in vitro and to observe their growth and differentiation. Method： NSCs were isolated from hippocampus of SD rat embryos（P16-P18） and cultured in DMEM/F12 medium containing EGF,bFGF,B27. To observe process of cell proliferation by microscope and identify cell types by immunocytochemical analyses after differentiation. Result： NSCs grew well in serum-free conditional medium and their cell bodies present transparent with good refraction at about eighth day. After differentiation, the cells demonstrated NSE and GFAP immunoreactive. Conclusion： NSCs were cultured well in serum-free conditional medium and they could be induced to differentiate into neurons and astrocytes in serum conditional medium.

关 键 词：胚鼠 无血清培养基 神经干细胞 分化 

分 类 号：R762[医药卫生—耳鼻咽喉科]