替比夫定(素比伏)体外抑制乙型肝炎病毒复制的研究  

Effect of telbivudine(sebivo)against HBV replication in vitro

作　　者：卢银平[1] 董继华[1] 刘朝[1] 陆蒙吉 杨东亮[3] 

机构地区：[1]华中科技大学同济医学院附属协和医院,武汉430022 [2]德国Essen大学病毒学研究所,essen45147 [3]华中科技大学同济医学院附属同济医院,武汉430022

出　　处：《传染病信息》2008年第4期235-237,共3页Infectious Disease Information

摘　　要：目的观察替比夫定体外对乙型肝炎病毒(HBV)复制的抑制作用。方法 HBV 复制子 pHBV1.3质粒转染 Huh7细胞,在转染细胞培养液中加入不同浓度的替比夫定,然后在不同时间段观察替比夫定的抗病毒作用。用 ELISA 检测培养上清中HBsAg、HBeAg 水平,用荧光定量 PCR(FQ-PCR)检测培养上清中 HBVDNA 拷贝数,用 Southern blot 分析转染细胞中 HBV 复制中间体。结果替比夫定减少了培养上清中 HBsAg、HBeAg 的表达及 HBV DNA 拷贝数,抑制了转染细胞中 HBV 复制中间体的形成。替比夫定对 HBV 复制子体外复制的抑制作用依赖于药物浓度及作用时间。结论替比夫定在体外对 HBV 有较强的抑制作用。Objective To observe effect of telbivudine, a novel antiviral nucleoside analogue, against HBV replication in vitro. Methods After HBV replicon pHBV 1.3 plasmid was transfected into Huh7 cell line, telbivudine was administered to culture medium with different concentration, then antiviral effect of telbivudine was observed in different periods of time. The levels of HBsAg and HBeAg in supernatant of Huh7 cells were measured by ELISA and the number of HBV DNA by FQ-PCR. Intracellular HBV replicative intermediates were analyzed by Southern blot. Results Telbivudine reduced the levels of HBsAg and HBeAg and the numberofHBV DNA in supernatant of Huh7 cells, and also decreased intraeellular HBV replicative intermediates. The inhibition effect of telbivudine against HBV replication in vitro depended on drug concentration and action time. Conclusion Telbivudine greatly inhibits HBV replication in Huh7 cells transfected from pHBV1.3.

关 键 词：乙型肝炎病毒 替比夫定 治疗 

分 类 号：R512.62[医药卫生—内科学]