HPLC检测人参皂苷在温和酸性条件下的降解变化  

Degradation of ginsenosides under mild acidic conditions monitored by high performance liquid chromatography

作　　者：冯仲扬[1,2] 张燮[1,2] 

机构地区：[1]大连大学医学检验系 [2]吉林大学化学系

出　　处：《中国药学杂志》1997年第12期772-774,共3页Chinese Pharmaceutical Journal

摘　　要：目的：在温和酸性条件下，对人参皂苷２０（Ｐ１）原人参二醇组中的两种主要单体皂苷Ｒｄ和Ｒｂ１酸水解情况进行研究。方法：水解产物采用高效液相色谱法（ＨＰＬＣ）标识。结果：人参皂苷Ｒｄ降解产生次级苷２０（Ｐ１）Ｒｇ３和Ｆ２；Ｒｂ１水解后，除以上两种次级苷２０（Ｐ１）Ｒｇ３和Ｆ２，主产物表明为Ｒｄ。结论：ＨＰＬＣ对人参皂苷在温和酸性条件下降解产物的检测结果是令人满意的，有助于了解皂苷的相互转化特性及其代谢机制。OBJECTIVE: To study the hydrolysis of ginsenoside Rd and Rb1, two kinds of main ginsenosides of the 20 (P1)protopanaxadiol group, under mild acidic conditions. METHOD: The hydrolysates were examined by high performance liquid chromatography (HPLC). RESULTS: Ginsenoside Rd was decomposed to yield the prosapogenins: 20 (P1)Rg3 and F2; the Rb1prosapogenins were Rd, 20 (P1)Rg3 and F2. CONCLUSION: The results of degradation reaction of ginsenoside under mild acidic conditions monitored by HPLC are acceptable. They will help as to learn changing characteristics and catabolic mechanism of ginsenosides.

关 键 词：人参皂苷 高效液相色谱 人参 

分 类 号：R284[医药卫生—中药学]