引起梨花枯和芽枯的Pseudomonas syringae pv.syringae病原细菌鉴定  

Identification of Pseudomonas syringae pv. syringae Causing Pear Blossom and Bud Blast in China

作　　者：邱文[1] 徐福寿[2] 谢关林[1] 徐丽慧[1] 怀雁[1] 李斌[1] 余山红[1] 钱军[2] 

机构地区：[1]浙江大学生物技术研究所,杭州310029 [2]杭州市植保土肥总站,杭州310020

出　　处：《中国农业科学》2008年第9期2657-2662,共6页Scientia Agricultura Sinica

基　　金：国家高技术研究与发展计划(“863”计划,2006AA10A211);国家自然科学基金(30671397);杭州市农业发展基金(2007-4)

摘　　要：【目的】明确在中国发生的梨花枯和芽枯的确切病原菌。【方法】用普通细菌学方法、电镜观察、Koch氏病原假说测定、Biolog、脂肪酸分析、PCR及与标准对照菌株的比较。【结果】从16个病样中分离获得12菌株,6株代表菌株显示出与Pseudomonas syringaepv.syringae3株标准对照菌株相似的致病反应,它们的Biolog和脂肪酸分析的相似度分别为0.57～0.86和0.58～0.81,PCR和序列测定结合上述结果证实了P.syringaepv.syringae为该病的病原菌。【结论】首次证实了中国梨树上的花枯和芽枯可由P.syringaepv.syringae引起。[Objective] This study was made to determine the causal organism of pear blossom and bud blast in China. [Method] The causal organism was identified by bacteriological test, electro- microscopic observation, Koch＇s postulation test, Biolog, fatty acid methyl esters （FAMEs） and PCR test and compared with the standard strains. [Result]Six representative strains out of 20 pathogenic bacterial isolates from 16 diseased samples showed characteristics similar to 3 standard strains of Pseudomonas syringae pv. syringae from Belgium. They were identified as P. syringae pv. syringae with Biolog similarity of 0.57-0.86 and FAMEs similarity of 0.58-0.81. The bacterium was reisolated from symptomatic plants and blossoms. Identification as P. syringae pv. syringae was confirmed by using PCR primers and sequences test and combined with the above results. [Conclusion] It has been confirmed that the pear blossom and bud blast in China may caused by P. syringae pv. syringae.

关 键 词：梨花枯 梨芽枯 PSEUDOMONAS SYRINGAE pv.Syringae 证实 

分 类 号：S436.612[农业科学—农业昆虫与害虫防治]