精氨酸对早期断奶仔猪肠道生长、组织形态及IL-2基因表达水平的影响  

Effect of Arginine on Development, Mucosal Morphology and IL-2 Gene Expression Levels of Digestive Tract in Early-Weaned Piglets

作　　者：谭碧娥[1] 李新国[1] 孔祥峰[1] 姚康[1] 印遇龙[1] 

机构地区：[1]中国科学院亚热带农业生态研究所动物生态营养与健康养殖联合实验室/农业生态工程重点实验室

出　　处：《中国农业科学》2008年第9期2783-2788,共6页Scientia Agricultura Sinica

基　　金：国家“973”项目(2004CB117502);国家自然科学基金(30771558,0700581);湖南省重大科技专项(2007FJ1003)

摘　　要：【目的】研究精氨酸对超早期断奶仔猪肠道发育和肠道免疫的影响。【方法】试验选用70头7日龄断奶的二元杂交仔猪,随机分成5个处理,每个处理14头,分别饲喂添加0.0%、0.2%、0.4%、0.6%和0.8%精氨酸的日粮。试验开始后第7天和第14天,每个处理分别选取6头猪屠宰取样,检测胃肠指数、小肠组织形态以及小肠IL-2基因表达水平。【结果】试验第7天,添加精氨酸一定程度上促进了胃和小肠以及小肠绒毛的生长(P>0.05),显著提高了空肠和回肠IL-2基因表达水平(P<0.05);试验第14天,添加0.6%和0.8%精氨酸显著提高仔猪小肠重量和长度(P<0.05),空肠和回肠绒毛高度以及绒毛高度与隐窝深度比值也均有提高的趋势(P>0.05)。【结论】精氨酸能促进超早期断奶仔猪的肠道发育,阻止肠绒毛萎缩,提高肠道IL-2基因表达水平,增强肠道免疫功能。[Objective] The study was conducted to evaluate the effects of arginine （Arg） on digestive tract development and intestinal immunity in piglets. [Method] A total of 70 piglets weaned at 7-day-old of age were assigned randomly into 5 treatment groups （14 pigs/group）, and they were fed with diets supplemented with 0.0% （control）, 0.2%, 0.4%, 0.6% and 0.8% L-arginine, respectively. On days 7 and 14 after initiation of the experiment, 6 piglets from each group were randomly sampled and slaughtered for determining index of stomachs and small intestines, morphology of intestinal mucosa, as well as intestinal IL-2 gene expression levels. [Result] The results showed that dietary supplementation with arginine improved （P〉0.05） the development of stomach, small intestine and villus, and remarkably increased （P〈0.05） the intestinal IL-2 gene expression levels on day 7. On day 14, the weight and length of small intestine were higher （P〈0.05）, and the villous height/crypt depth in jejunum and ileum had the tendency to be higher （P〉0.05） in piglets supplemented with 0.6%, 0.8% arginine than those in control group. Collectively, these findings indicate that arginine supplementation may improve the development of digestive tract, prevent intestinal villous atrophy, increase the levels of intestinal IL-2 gene expression and enhance the immune status in early-weaned piglets.

关 键 词：精氨酸 超早期断奶仔猪 肠道发育 组织形态 IL-2基因表达 

分 类 号：S828[农业科学—畜牧学]