抗肿瘤坏死因子抗体对体外循环肺组织超微结构的影响  

Intratracheal anti-tumor necrosis factor-α antibody effect on ultra-structure of lung after cardiopulmonary bypass

作　　者：于洋[1] 李琴[1] 胡晖[1] 刘晓[1] 

机构地区：[1]首都医科大学附属北京安贞医院心脏外科,北京100029

出　　处：《中国综合临床》2008年第10期977-979,共3页Clinical Medicine of China

基　　金：北京市优秀人才培养资助基金项目（20061D0300600D84）

摘　　要：目的探讨气管内给予抗肿瘤坏死因子抗体（TNF—α Ab）对体外循环（CPB）肺损伤组织超微结构的影响。方法选取健康家兔28只，随机分成4组，每组7只，即：I组为单纯开胸组；Ⅱ组为单纯CPB组；Ⅲ组为CPB＋经气管插管注入生理盐水组；Ⅳ组为CPB＋TNF-α Ab组。给药方法是于术前和主动脉开放后经气管插管注入抗兔TNF—α Ab（总量2400pg／kg）。采取肺组织样本，电镜观察其超微结构，并对TNF-α mRNA和TNF—α蛋白的表达及细胞凋亡情况进行观察。结果TNF-α Ab明显抑制了肺源性TNF—α的释放，降低了肺含水量，减少了肺组织细胞凋亡的发生，且病理形态学改变较轻。结论气管内应用TNF-α Ab能明显减轻体外循环时肺组织超微结构的损伤。Objective To study the effect of intratracheal anti-tumor necrosis factor-α antibody （ TNF-α Ab） on ultra-structure of lung after cardiopulmonary bypass（CPB）. Method 28 healthy rabbits were selected and randomly evenly divided into four groups： Ⅰ group only received open chest operation; Ⅱ -Ⅳ groups underwent CPB. In the Ⅳ group,rabbit TNF-α Ab （2400 pg/kg） was dropped into the intracheal tube before operation and just after releasing the aortic clamp. Saline was given to the Ⅲ group by the same way. Water volume,TNF-α mRNA,TNF-α protein,apoptosis and pathomorphological changes were measured in the lung tissues. Results TNF-α Ab can reduce releasing of TNF-α. It could also reduce the occurrence of apoptosis and attenuate pathomorphological changes in the lung tissue. Conclusion Intratraoheal TNF-α Ab markedly lessenes the injury of uhrastructure of lung after CPB.

关 键 词：体外循环 抗肿瘤坏死因子抗体 肺损伤 肺保护 细胞凋亡 

分 类 号：R654.1[医药卫生—外科学]